Filter: AllWBIPIHC

WB analysis of Jurkat using 17958-1-AP (same clone as 17958-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
WB analysis of A375 using 17958-1-AP (same clone as 17958-1-PBS)
A375 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
WB analysis of MKN-45 using 17958-1-AP (same clone as 17958-1-PBS)
MKN-45 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
WB analysis of SGC-7901 using 17958-1-AP (same clone as 17958-1-PBS)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 17958-1-AP (same clone as 17958-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
IP experiment of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)
IP result of anti-LOX (IP:17958-1-AP, 3ug; Detection:17958-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.
IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

LOX Polyclonal antibody, PBS Only

LOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 17958-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Protein-lysine 6-oxidase, short form, Protein-lysine 6-oxidase, long form, Protein-lysine 6-oxidase, lysyl oxidase, EC:1.4.3.13

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

17958-1-PBS targets LOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12479 Product name: Recombinant human LOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC074872 Sequence: MRFAWTVLLLGPLQLCALVHCAPPAAGQQQPPREPPAAPGAWRQQIQWENNGQVFSLLSLGSQYQPQRRRDPGAAVPGAANASAQQPRTPILLIRDNRTAAARTRTAGSSGVTAGRPRPTARHWFQAGYSTSRAREAGASRAENQTAPGEVPALSNLRPPSRVDGMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGGRYRPGYGTGYFQYGLPDLVADPYYIQASTYVQKMSMYNLRCAAEENCLASTAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYGADIDCQWIDITDVKPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY Predict reactive species
Full Name	lysyl oxidase
Calculated Molecular Weight	417 aa, 47 kDa
Observed Molecular Weight	32 kDa, 45-47 kDa
GenBank Accession Number	BC074872
Gene Symbol	LOX
Gene ID (NCBI)	4015
RRID	AB_2234541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28300
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.