Tested Applications
| Positive WB detected in | Jurkat cells, MKN-45 cells, SGC-7901 cells, NIH/3T3 cells, mouse skeletal muscle tissue, A375 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17958-1-AP targets LOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12479 Product name: Recombinant human LOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-417 aa of BC074872 Sequence: MRFAWTVLLLGPLQLCALVHCAPPAAGQQQPPREPPAAPGAWRQQIQWENNGQVFSLLSLGSQYQPQRRRDPGAAVPGAANASAQQPRTPILLIRDNRTAAARTRTAGSSGVTAGRPRPTARHWFQAGYSTSRAREAGASRAENQTAPGEVPALSNLRPPSRVDGMVGDDPYNPYKYSDDNPYYNYYDTYERPRPGGRYRPGYGTGYFQYGLPDLVADPYYIQASTYVQKMSMYNLRCAAEENCLASTAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLDANTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYGADIDCQWIDITDVKPGNYILKVSVNPSYLVPESDYTNNVVRCDIRYTGHHAYASGCTISPY Predict reactive species |
| Full Name | lysyl oxidase |
| Calculated Molecular Weight | 417 aa, 47 kDa |
| Observed Molecular Weight | 32 kDa, 45-47 kDa |
| GenBank Accession Number | BC074872 |
| Gene Symbol | LOX |
| Gene ID (NCBI) | 4015 |
| RRID | AB_2234541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28300 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LOX antibody 17958-1-AP | Download protocol |
| IP protocol for LOX antibody 17958-1-AP | Download protocol |
| WB protocol for LOX antibody 17958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LOX (Lysyl Oxidase) antibody, catalog number 17958-1-AP, has 55 total citations. These appear in journals including Signal Transduction and Targeted Therapy, Oncogene, Redox Biology, Advanced Healthcare Materials, BMC Medicine, Science Reports, Journal of Molecular and Cellular Cardiology, and others. Research fields span myocardial fibrosis, cancer metastasis (lung, breast, gastric, ovarian, colorectal), pulmonary fibrosis, ferroptosis, extracellular matrix remodeling, copper metabolism, drug resistance, and tissue repair.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther 2-APQC, a small-molecule activator of Sirtuin-3 (SIRT3), alleviates myocardial hypertrophy and fibrosis by regulating mitochondrial homeostasis | ||
Bioact Mater Reconstructing protein conformations to activate follicular enzyme-nutrient reservoirs for reversing follicle aging. | ||
Redox Biol Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer | ||
Adv Healthc Mater Enhanced Vascular Smooth Muscle Cell and Extracellular Matrix Repair Using a Metal-Organic Framework-Based Co-Delivery System for Abdominal Aortic Aneurysm Therapy | ||
J Orthop Translat Hydrogen sulfide generated by cystathionine gamma lyase inhibits lysyl oxidase and protects against calcific tendinopathy. | ||
Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids |
Reviews
What customers say
Both reviews are from the same user, who gave 5-star ratings for Western Blot applications on liver tissue using a 1:1000 dilution. The reviewer consistently praised the antibody as "best for Western Blot," indicating strong satisfaction with its performance in this application. No negative feedback or issues were reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-07-2025) | BEST FOR WESTERN
|
MALLIKARJUNA (Verified Customer) (11-07-2025) | BEST FOR WESTREN BLOT
|
