CoraLite® Plus 488-conjugated LOXL2 Monoclonal antibody

LOXL2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7F1

Cat no : CL488-67139

Synonyms

LOR2, LOXL2, Lysyl oxidase homolog 2, lysyl oxidase like 2, Lysyl oxidase like protein 2, WS9 14

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Validation Data Gallery

• 

  WB analysis using CL488-67139

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67139 (LOXL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67139 targets LOXL2 in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LOXL2 fusion protein Ag28566
• Full Name: lysyl oxidase-like 2
• Calculated Molecular Weight: 774 aa, 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000594
• Gene Symbol: LOXL2
• Gene ID (NCBI): 4017
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).