LPCAT4 Polyclonal antibody

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  WB analysis using 17905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17905-1-AP (LPCAT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17905-1-AP

  WB result of LPCAT4 antibody (17905-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPCAT4 transfected HeLa cells.

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

LPCAT4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17905-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1-AGP acyltransferase 7, 1-acylglycerophosphoserine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerol-3-phosphate O-acyltransferase 7, 1 AGPAT 7

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Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

17905-1-AP targets LPCAT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12281

  Product name: Recombinant human LPCAT4 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 175-524 aa of BC092463

  Sequence: PASRRRVVEEVRRRATSGGKWPQVLFFPEGTCSNKKALLKFKPGAFIAGVPVQPVLIRYPNSLDTTSWAWRGPGVLKVLWLTASQPCSIVDVEFLPVYHPSPEESRDPTLYANNVQRVMAQALGIPATECEFVGSLPVIVVGRLKVALEPQLWELGKVLRKAGLSAGYVDAGAEPGRSRMISQEEFARQLQLSDPQTVAGAFGYFQQDTKGLVDFRDVALALAALDGGRSLEELTRLAFELFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPAATALHAELCQAGSSQGLSLCQFQNFSLHDPLYGKLFSTYLRPPHTSRGTSQTPNASSPGNPTALANGTVQAPKQKGD

• Full Name: lysophosphatidylcholine acyltransferase 4
• Calculated Molecular Weight: 524 aa, 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC092463
• Gene Symbol: LPCAT4
• Gene ID (NCBI): 254531
• RRID: AB_2140478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q643R3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPCAT4, also known as lysophosphatidylcholine acyltransferase 4, is an enzyme that plays a crucial role in lipid metabolism and membrane remodeling It is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family and is involved in the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction. The protein is predicted to be located in the membrane and is classified as a metabolic protein and enzyme. It is involved in biological processes such as lipid biosynthesis, lipid metabolism, phospholipid biosynthesis, and phospholipid metabolism. .

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Hepatol; Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.; Authors - Morita Yoshifumi Y
mouse	WB	Free Radic Biol Med; Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury; Authors - Wei Deng
mouse	WB	Neurochem Res; iPLA2β Knockout Mouse, a Genetic Model for Progressive Human Motor Disorders, Develops Age-Related Neuropathology.; Authors - Helene Blanchard
human	WB	FASEB J; Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas' disease progression; Authors - Chunhua Liu
human	WB	Front Biosci (Landmark Ed); Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation; Authors - Huiyuan Gong
mouse	WB	FASEB J; Overexpression of lysophospholipid acyltransferase, LPLAT10/LPCAT4/LPEAT2, in the mouse liver increases glucose-stimulated insulin secretion; Authors - Kahori Shimizu