LPL Polyclonal antibody

LPL Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16899-1-AP

Synonyms

HDLCQ11, LIPD, lipoprotein lipase, LPL

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 16899-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16899-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 16899-1-AP is LPL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LPL fusion protein Ag10431
• Full Name: lipoprotein lipase
• Calculated Molecular Weight: 475 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011353
• Gene Symbol: LPL
• Gene ID (NCBI): 4023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPL(lipoprotein lipase) is also named as LIPD and belongs to the AB hydrolase superfamily. LPL catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins. It plays a role in the binding of lipoprotein particles to cell-surface molecules, including sulfated glycosaminoglycans (GAGs)(PMID:21177248). The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. This protein is highly glycosylated and functions as a head-to-tail dimer under well-defined conditions(PMID:11154699).

Publications

Species	Application	Title
mouse	WB	Diabetes; Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage.; Authors - Qiaoli Chen
		Diabetes; Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.; Authors - Xinyu Yang