LPP Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP02282-2

  Sandwich ELISA standard curve of MP02282-2, Human LPP Recombinant Matched Antibody Pair - PBS only. 86117-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19205. 86117-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02282-1

  The mean LPP concentration was determined to be 30.5 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

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  Sample test of MP02282-2

  The mean LPP concentration was determined to be 43,872.1 pg/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load and 4,111.8 pg/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP02282-1

  Cytometric bead array standard curve of MP02282-1, LPP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86117-2-PBS. Detection antibody: 86117-1-PBS. Standard: Ag19205. Range: 0.156-20 ng/mL.

LPP Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86117-2-PBS

Clone No.250773F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

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Product Information

86117-2-PBS targets LPP as part of a matched antibody pair:

MP02282-1: 86117-2-PBS capture and 86117-1-PBS detection (validated in Cytometric bead array)

MP02282-2: 86117-2-PBS capture and 86117-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19205

  Product name: Recombinant human LPP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC130584

  Sequence: MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPRPPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASYTTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPGLQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG

• Full Name: LIM domain containing preferred translocation partner in lipoma
• Calculated Molecular Weight: 612 aa, 66 kDa
• GenBank Accession Number: BC130584
• Gene Symbol: LPP
• Gene ID (NCBI): 4026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93052
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.