LRCH1 Polyclonal antibody
LRCH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 25017-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 25017-1-AP is LRCH1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LRCH1 fusion protein Ag18728 |
Full Name | leucine-rich repeats and calponin homology (CH) domain containing 1 |
Calculated molecular weight | 728 aa, 80 kDa |
Observed molecular weight | 85-95 kDa |
GenBank accession number | BC112937 |
Gene symbol | LRCH1 |
Gene ID (NCBI) | 23143 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.
Protocols
Product Specific Protocols | |
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WB protocol for LRCH1 antibody 25017-1-AP | Download protocol |
IHC protocol for LRCH1 antibody 25017-1-AP | Download protocol |
IF protocol for LRCH1 antibody 25017-1-AP | Download protocol |
IP protocol for LRCH1 antibody 25017-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |