LRCH1 Polyclonal antibody

LRCH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25017-1-AP

Synonyms

CHDC1, KIAA1016, LRCH1, Neuronal protein 81

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Validation Data Gallery

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  WB analysis of HeLa using 25017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25017-1-AP

  IP Result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of mouse cerebellum using 25017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 25017-1-AP

  Immunofluorescent analysis of MCF-7 cells using 25017-1-AP (LRCH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25017-1-AP is LRCH1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRCH1 fusion protein Ag18728
• Full Name: leucine-rich repeats and calponin homology (CH) domain containing 1
• Calculated molecular weight: 728 aa, 80 kDa
• Observed molecular weight: 85-95 kDa
• GenBank accession number: BC112937
• Gene symbol: LRCH1
• Gene ID (NCBI): 23143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.