LRCH1 Antibody

LRCH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25017-1-AP

Synonyms

CHDC1, KIAA1016, LRCH1, Neuronal protein 81



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25017-1-AP is LRCH1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRCH1 fusion protein Ag18728
Full Name leucine-rich repeats and calponin homology (CH) domain containing 1
Calculated molecular weight 728 aa, 80 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC112937
Gene symbol LRCH1
Gene ID (NCBI) 23143
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.

Protocols

Product Specific Protocols
WB protocol for LRCH1 antibody 25017-1-APDownload protocol
IHC protocol for LRCH1 antibody 25017-1-APDownload protocol
IF protocol for LRCH1 antibody 25017-1-APDownload protocol
IP protocol for LRCH1 antibody 25017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols