LRCH1 Polyclonal antibody

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  WB analysis of HeLa using 25017-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25017-1-AP (LRCH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25017-1-AP

  IP result of anti-LRCH1 (IP:25017-1-AP, 4ug; Detection:25017-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of mouse cerebellum using 25017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25017-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25017-1-AP (LRCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 25017-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using LRCH1 antibody (25017-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LRCH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25017-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Neuronal protein 81, Leucine-rich repeat and calponin homology domain-containing protein 1, KIAA1016, CHDC1, Calponin homology domain-containing protein 1

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25017-1-AP targets LRCH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18728

  Product name: Recombinant human LRCH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 353-549 aa of BC112937

  Sequence: NIMEEEQIIKEDSCHRLSPVKGEFHQEFQPEPSLLGDSTNSGEERDQFTDRADGLHSEFMNYKARAEDCEELLRIEEDVHWQTEGIISSSKDQDMDIAMIEQLREAVDLLQDPNGLSTDITERSVLNLYPMGSAEALELQDSALNGQIQLETSPVCEVQSDLTLQSNGSQYSPNEIRENSPAVSPTTNSTAPFGLKP

• Full Name: leucine-rich repeats and calponin homology (CH) domain containing 1
• Calculated Molecular Weight: 728 aa, 80 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC112937
• Gene Symbol: LRCH1
• Gene ID (NCBI): 23143
• RRID: AB_2879848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2L9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.