LY96/MD2 Polyclonal antibody

LY96/MD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11784-1-AP

Synonyms

ESOP 1, ESOP1, Ly 96, LY96, lymphocyte antigen 96, MD 2, MD2, Protein MD 2

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Validation Data Gallery

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  WB analysis of U-937 using 11784-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11784-1-AP (LY96/MD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

11784-1-AP targets LY96/MD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LY96/MD2 fusion protein Ag2447
• Full Name: lymphocyte antigen 96
• Calculated molecular weight: 160 aa, 18 kDa
• Observed molecular weight: 20 kDa and 30 kDa and 35 kDa
• GenBank accession number: BC020690
• Gene symbol: LY96
• Gene ID (NCBI): 23643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LY96, also named as ESOP1 or MD 2, is a small secreted glycoprotein that can bind to both the hydrophobic portion of LPS and to the extracellular domain of TLR4. The interaction between MD-2 and LPS bridges the two TLR4 molecules and induces the dimerization of LPS-MD-2-TLR4, which forms the structural basis for biological functions of TLR4/MD-2 complex. Due to its essential role in mediating the interaction between LPS and TLR4, MD-2 has been extensively explored as a therapeutic target for treatment of inflammatory disorders.

Publications

Species	Application	Title
human	WB	Oncol Rep; Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways.; Authors - Lanfeng Zhang
rat		Molecules; Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome.; Authors - Yang Hu
mouse	WB	J Cardiovasc Transl Res; Macrophage CD36 and TLR4 Cooperation Promotes Foam Cell Formation and VSMC Migration and Proliferation Under Circadian Oscillations.; Authors - Zhen Sun
human	WB	Front Oncol; MD2 Is a Potential Biomarker Associated with Immune Cell Infiltration in Gliomas.; Authors - Mengya Zhao