LYPD3 Recombinant antibody

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  WB analysis using 83345-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83345-1-RR (LYPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83345-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83345-1 -RR against Human LYPD3 were performed. The affinity constant is 3.04 nM.

LYPD3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83345-1-RR

Clone No.240347C5

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MIG C4, LY6/PLAUR domain containing 3, C4.4A, 240347C5

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Tested Applications

• Positive WB detected in: MCF-7 cells, HaCaT cells, SK-BR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

83345-1-RR targets LYPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LYPD3 fusion protein Ag34961
• Full Name: LY6/PLAUR domain containing 3
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: NM_014400
• Gene Symbol: LYPD3
• Gene ID (NCBI): 27076
• RRID: AB_3671004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYPD3 (Ly6/PLAUR domain-containing protein 3), also known as C4.4A , is a membrane protein anchored to the cell surface via a glycosylphosphatidylinositol (GPI) moiety (PMID: 25302166; 15012588). It is a tumorigenic and high-glycosylated protein that has been proven to be linked with the carcinogenic effects in different solid tumors, such as lung cancer and breast cancer (PMID: 32040344; 29371917; 31644911). It has been shown that the elevated expression of LYPD3 is associated with lung adenocarcinoma carcinogenesis and poor prognosis (PMID: 25302166; 32040344).

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; Unveiling Varied Cell Death Patterns in Lung Adenocarcinoma Prognosis and Immunotherapy Based on Single-Cell Analysis and Machine Learning; Authors - Zipei Song