Mouse CSF1R/CD115 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84919-1-RR (same clone as 84919-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.

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  WB analysis using 84919-1-RR (same clone as 84919-1-PBS)

  J774A.1 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 84919-1-RR (same clone as 84919-1-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01676-2

  Sandwich ELISA standard curve of MP01676-2, Mouse CSF1R/CD115 Recombinant Matched Antibody Pair - PBS only. 84919-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1816. 84919-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01676-2

  Serum of eight mice was measured. The CSF1R/CD115 concentration of detected samples was determined to be 2.9 ug/mL with a range of 2.3-3.7 ug/mL.

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  Sample test of MP01676-2

  RAW 264.7were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CSF1R/CD115, and measured 1,189.8 pg/mL.

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  Sample test of MP01676-2

  The mean CSF1R/CD115 concentration was determined to be 760.8 ng/mL in RAW 264.7 cell extract based on a 7.5 mg/mL extract load.

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  IF Staining of RAW 264.7 using 84919-1-RR (same clone as 84919-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-1-RR, Clone: 242213B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01676-2

  Cytometric bead array standard curve of MP01676-2, MOUSE CSF1R/CD115 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84919-1-PBS. Detection antibody: 84919-2-PBS. Standard: Eg1816. Range: 0.625-80 ng/mL.

CSF1R/CD115 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84919-1-PBS

Clone No.242213B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Csf1r, Csfmr, CSF-1 receptor, CSF 1R, CD115

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Product Information

84919-1-PBS targets CSF1R/CD115 as part of a matched antibody pair:

MP01676-2: 84919-1-PBS capture and 84919-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 1 receptor
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 110-170 kDa
• GenBank Accession Number: NM_001037859.2
• Gene Symbol: CSF1R/CD115
• Gene ID (NCBI): 12978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09581
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).