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  • Western Blot (WB) analysis of various lysates using CSF1R/CD115 Recombinant antibody (84919-1-RR)

    WB analysis using 84919-1-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CSF1R/CD115 Recombinant antibody (84919-1-RR)

    WB analysis using 84919-1-RR

    J774A.1 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CSF1R/CD115 Recombinant antibody (84919-1-RR)

    WB analysis of mouse spleen using 84919-1-RR

    mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CSF1R/CD115 Recombinant antibody (84919-1-RR)

    IF Staining of RAW 264.7 using 84919-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-1-RR, Clone: 242213B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CSF1R/CD115 Recombinant antibody

CSF1R/CD115 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84919-1-RR
Clone No.242213B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Csf1r, Csfmr, CSF-1 receptor, CSF 1R, CD115

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inRAW 264.7 cells, J774A.1 cells, mouse spleen tissue
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84919-1-RR targets CSF1R/CD115 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name colony stimulating factor 1 receptor
Calculated Molecular Weight109 kDa
Observed Molecular Weight110-170 kDa
GenBank Accession NumberNM_001037859.2
Gene Symbol CSF1R/CD115
Gene ID (NCBI) 12978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Protocols

Product Specific Protocols
WB protocol for CSF1R/CD115 antibody 84919-1-RRDownload protocol
IF protocol for CSF1R/CD115 antibody 84919-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols