MAdCAM1 Polyclonal antibody

MAdCAM1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21917-1-AP

Synonyms

hMAdCAM 1, MACAM1, MAdCAM1

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Validation Data Gallery

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  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MAdCAM1 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 21917-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21917-1-AP (MADCAM1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21917-1-AP is MAdCAM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAdCAM1 fusion protein Ag16184
• Full Name: mucosal vascular addressin cell adhesion molecule 1
• Calculated Molecular Weight: 406 aa, 43 kDa
• GenBank Accession Number: BC142629
• Gene Symbol: MADCAM1
• Gene ID (NCBI): 8174
• RRID: AB_2802114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Publications

Species	Application	Title
	IF	Immunity; T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.; Authors - Ya-Yuan Fu
	WB	Oncotarget; Cytological study on the regulation of lymphocyte homing in the chicken spleen during LPS stimulation.; Authors - Qian Zhang
human	WB,IHC	Cell Death Dis; Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer.; Authors - Jieyun Zhang