MAGEB18 Polyclonal antibody

MAGEB18 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17494-1-AP

Synonyms

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18

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Validation Data Gallery

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  WB analysis using 17494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17494-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 17494-1-AP

  Immunofluorescent analysis of A549 cells using 17494-1-AP (MAGEB18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, A549 cells, MCF-7 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17494-1-AP targets MAGEB18 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEB18 fusion protein Ag11624
• Full Name: melanoma antigen family B, 18
• Calculated Molecular Weight: 343 aa, 39 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC029525
• Gene Symbol: MAGEB18
• Gene ID (NCBI): 286514
• RRID: AB_2138404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.