Validation Data Gallery
|Positive WB detected in
|K-562 cells, HeLa cells, HL-60 cells, human brain tissue, Raji cells
|Positive IP detected in
|Positive IHC detected in
|human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12347-1-AP targets MAGOH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|MAGOH fusion protein Ag3004
|mago-nashi homolog, proliferation-associated (Drosophila)
|Calculated molecular weight
|146 aa, 17 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.
NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.
The exon junction complex component Magoh controls brain size by regulating neural stem cell division.
Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly.
Dosage-dependent requirements of Magoh for cortical interneuron generation and survival.
Generation of a Magoh conditional allele in mice.
J Exp Zool B Mol Dev Evol
Mago, a vertebrate homolog of Drosophila Mago nashi protein, is a component of the chromatoid body in the cytoplasm of the postmeiotic spermatid.