• Featured Product
  • KD/KO Validated

LC3 Polyclonal antibody

LC3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14600-1-AP

Synonyms

LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B



Tested Applications

Positive WB detected inChloroquine treated HepG2 cells, mouse brain tissue, mouse lung tissue, mouse testis tissue, human skeletal muscle tissue, rat brain tissue, Chloroquine treated HEK-293 cells, mouse heart tissue, mouse liver tissue, HeLa cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, human testis tissue, mouse brain tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HeLa cells, Chloroquine treated HepG2 cells, Chloroquine treated U2OS cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14600-1-AP targets LC3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, monkey, rabbit, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LC3 fusion protein Ag6144
Full Name microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight 15 kDa
Observed molecular weight 14-18 kDa
GenBank accession numberBC067797
Gene symbol MAP1LC3B
Gene ID (NCBI) 81631
RRIDAB_2137737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protocols

Product Specific Protocols
WB protocol for LC3 antibody 14600-1-APDownload protocol
IHC protocol for LC3 antibody 14600-1-APDownload protocol
IF protocol for LC3 antibody 14600-1-APDownload protocol
IP protocol for LC3 antibody 14600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
mouseWB

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
mouseIF,WB

Bioact Mater

Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway

Authors - Tian Qin
humanWB

Acta Neuropathol

C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy.

Authors - Christopher P Cali
humanWB

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-02-2024)

Good antibody, single band at the predicted molecular weight, no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y lysate
FH

Hala (Verified Customer) (05-09-2023)

works very good in milk5%

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HepG2
LC3 Antibody Western Blot validation (1/2000 dilution) in HepG2 (Cat no:14600-1-AP)
FH

Hala (Verified Customer) (04-28-2023)

works very good with milk blocking

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HepG2
FH

Zhihao (Verified Customer) (07-25-2022)

We could detect one form of LC3 in our cell samples, which should be two.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: GFP-Parkin transfected HeLa cell
LC3 Antibody Western Blot validation (1:1000 dilution) in GFP-Parkin transfected HeLa cell (Cat no:14600-1-AP)
FH

S (Verified Customer) (12-31-2021)

I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HCT116
LC3 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:14600-1-AP)
FH

YING (Verified Customer) (09-25-2021)

The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: mouse cells
LC3 Antibody Western Blot validation (1:1500 dilution) in mouse cells (Cat no:14600-1-AP)
FH

Eric (Verified Customer) (03-13-2021)

Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia
LC3 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia (Cat no:14600-1-AP)
FH

Elzbieta (Verified Customer) (09-23-2020)

The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver, human HepG2 cells
FH

An (Verified Customer) (09-17-2020)

Used for IHC on zebrafish cryosections of eyes. Worked well.

  • Applications: Immunohistochemistry
FH

David (Verified Customer) (03-30-2020)

Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y