|Positive WB detected in||Chloroquine treated HepG2 cells, HEK-293 cells, Chloroquine treated HEK-293 cells, HeLa cells, HSC-T6 cells, human skeletal muscle tissue, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
14600-1-AP targets LC3 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, duck, goat, human, monkey, mouse, pig, rabbit, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LC3 fusion protein Ag6144|
|Full Name||microtubule-associated protein 1 light chain 3 beta|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||14-18 kDa|
|GenBank accession number||BC067797|
|Gene ID (NCBI)||81631|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
|Product Specific Protocols|
|WB protocol for LC3 antibody 14600-1-AP||Download protocol|
|IHC protocol for LC3 antibody 14600-1-AP||Download protocol|
|IF protocol for LC3 antibody 14600-1-AP||Download protocol|
|IP protocol for LC3 antibody 14600-1-AP||Download protocol|
|FC protocol for LC3 antibody 14600-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Eric (Verified Customer) (03-13-2021)
Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.
Elzbieta (Verified Customer) (09-23-2020)
The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.
An (Verified Customer) (09-17-2020)
Used for IHC on zebrafish cryosections of eyes. Worked well.
David (Verified Customer) (03-30-2020)
Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.