MEK1/2 Polyclonal antibody

MEK1/2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Publications

48

Cat no : 11049-1-AP

Synonyms

ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 1/2, MEK 2, MEK1/2, MEK2, MKK2, PRKMK2

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Validation Data Gallery

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  WB analysis using 11049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 11049-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11049-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11049-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 11049-1-AP

  IP result of anti-MEK1/2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug.

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 11049-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (11049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 11049-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, HL-60 cells, Jurkat cells, SH-SY5Y cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Jurkat cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 47 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11049-1-AP targets MEK1/2 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEK1/2 fusion protein Ag1481
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MAP2K2
• Gene ID (NCBI): 5605
• RRID: AB_2140649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

Publications

Species	Application	Title
mouse	WB	Nat Commun; ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.; Authors - Yan Wang
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li
mouse,human	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou
human	WB	Cell Death Dis; The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.; Authors - Wenjuan Li
human	WB	Int J Mol Sci; Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling; Authors - Xinglian Xu