MAP4K5 Polyclonal antibody

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  WB analysis of mouse ovary using 12827-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12827-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12827-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 12827-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 12827-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12827-1-AP (MAP4K5 antibody) at dilution of 1:100 (under 10x lens).

MAP4K5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12827-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5

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Tested Applications

• Positive WB detected in: mouse ovary tissue, HeLa cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP4K5 fusion protein Ag3547
• Full Name: mitogen-activated protein kinase kinase kinase kinase 5
• Calculated Molecular Weight: 846 aa, 95 kDa
• Observed Molecular Weight: 58 kDa, 95 kDa, 120 kDa
• GenBank Accession Number: BC036013
• Gene Symbol: MAP4K5
• Gene ID (NCBI): 11183
• RRID: AB_2140986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4K4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.