MAPK10 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAPK10 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17572-1-AP

Synonyms

c Jun N terminal kinase 3, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, p493F12, p54bSAPK, PRKM10

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Validation Data Gallery

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  WB analysis of mouse brain using 17572-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17572-1-AP (MAPK10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17572-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17572-1-AP (MAPK10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 17572-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAPK10 antibody 17572-1-AP at 1:25 diluiton and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

17572-1-AP targets MAPK10 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPK10 fusion protein Ag11785
• Full Name: mitogen-activated protein kinase 10
• Calculated molecular weight: 464 aa, 53 kDa
• Observed molecular weight: 45-48 kDa, 54-57 kDa
• GenBank accession number: BC065516
• Gene symbol: MAPK10
• Gene ID (NCBI): 5602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Anat Rec (Hoboken); Inhibition of JNK3 Promotes Apoptosis Induced by BH3 Mimetic S1 in Chemoresistant Human Ovarian Cancer Cells.; Authors - Xiaochun Yang
rat	WB	Behav Brain Res; The potential role of HO-1 in regulating the MLK3-MKK7-JNK3 module scaffolded by JIP1 during cerebral ischemia/reperfusion in rats.; Authors - Yuan-Jian Song
human	WB,IHC	Onco Targets Ther; miR-137-3p Modulates the Progression of Prostate Cancer by Regulating the JNK3/EZH2 Axis.; Authors - Yachen Zang ; KD Validated
human	WB	Biomed Pharmacother; Icariside II suppresses cervical cancer cell migration through JNK modulated matrix metalloproteinase-2/9 inhibition in vitro and in vivo.; Authors - Ya-Sai Sun