MAPKAPK2 Polyclonal antibody

MAPKAPK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 13949-1-AP

Synonyms

MAPKAP kinase 2, MAPKAPK 2, MAPKAPK2, MK2

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Validation Data Gallery

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  WB analysis using 13949-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13949-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13949-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13949-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); F-actin is stained using CL555-phalloidin (red).

• 

  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse skeletal muscle tissue, mouse colon tissue
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

13949-1-AP targets MAPKAPK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPKAPK2 fusion protein Ag5060
• Full Name: mitogen-activated protein kinase-activated protein kinase 2
• Calculated Molecular Weight: 400 aa, 46 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC036060
• Gene Symbol: MAPKAPK2
• Gene ID (NCBI): 9261
• RRID: AB_2877995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385).

Publications

Species	Application	Title
human	WB	Mol Cancer Res; Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2.; Authors - Rui Wang
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Toxicol Appl Pharmacol; p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease.; Authors - Chen Gui
human	WB	Int J Mol Med; Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway.; Authors - Qimeng Wang
mouse	WB	Brain Res Bull; The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain.; Authors - Kai-Ge Ma
mouse	WB	Cell Rep; MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal.; Authors - Yan Zhang