MASP1 Polyclonal antibody

• 

  WB analysis of HeLa using 21837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21837-1-AP (MASP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 21837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21837-1-AP (MASP1 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

MASP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21837-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Complement factor MASP 3, CRARF, CRARF1, MASP, MASP 1, MASP1, PRSS5, RaRF, Serine protease 5

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

21837-1-AP targets MASP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16432

  Product name: Recombinant human MASP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 379-728 aa of BC106945

  Sequence: TFSTRNNLTTYKSEIKYSCQEPYYKMLNNNTGIYTCSAQGVWMNKVLGRSLPTCLPECGQPSRSLPSLVKRIIGGRNAEPGLFPWQALIVVEDTSRVPNDKWFGSGALLSASWILTAAHVLRSQRRDTTVIPVSKEHVTVYLGLHDVRDKSGAVNSSAARVVLHPDFNIQNYNHDIALVQLQEPVPLGPHVMPVCLPRLEPEGPAPHMLGLVAGWGISNPNVTVDEIISSGTRTLSDVLQYVKLPVVPHAECKTSYESRSGNYSVTENMFCAGYYEGGKDTCLGDSGGAFVIFDDLSQRWVVQGLVSWGGPEECGSKQVYGVYTKVSNYVDWVWEQMGLPQSVVEPQVER

• Full Name: mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
• Calculated Molecular Weight: 728 aa, 82 kDa
• Observed Molecular Weight: 40-70 kDa
• GenBank Accession Number: BC106945
• Gene Symbol: MASP1
• Gene ID (NCBI): 5648
• RRID: AB_2878924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48740
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Front Immunol; Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation; Authors - Binshan Zhang
human	IHC	Sci Rep; Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies; Authors - Yang Yang
human	WB	Discov Oncol; MASP1 in stomach adenocarcinoma: linking diagnosis, prognosis, and tumor immunity; Authors - Chuyu Xiao
human,mouse	WB,IHC,IP	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu ; KD Validated