MBOAT1 Polyclonal antibody

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  WB analysis using 25615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25615-1-AP (MBOAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MBOAT1 Polyclonal Antibody for WB, ELISA

Cat No. 25615-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LPEAT1, LPLAT 1, LPSAT, Lyso PS acyltransferase, MBOAT1, OACT1

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Tested Applications

• Positive WB detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25615-1-AP targets MBOAT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBOAT1 fusion protein Ag22470
• Full Name: membrane bound O-acyltransferase domain containing 1
• Calculated Molecular Weight: 495 aa, 57 kDa
• Observed Molecular Weight: 45-57 kDa
• GenBank Accession Number: BC150652
• Gene Symbol: MBOAT1
• Gene ID (NCBI): 154141
• RRID: AB_3085808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZNC8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBOAT1 (membrane bound O-acyltransferase domain containing 1), also known as LPLAT or LPSAT. It is expected to be located in membrane and widely expressed in colon, small intestine. MBOAT1 belongs to the membrane-bound O- acetyltransferase superfamily. Its encoded transmembrane protein is an enzyme that transfers organic compounds, preferably oleoyl-CoA, to the hydroxyl group of protein target in the membrane. The translocation that destroys the gene may be related to short finger toe syndrome. At the same time, it may also play a role in neurite growth during neuronal differentiation (PMID: 18772128). Studies have determined that phospholipid modifying enzymes MBOAT1 and MBOAT2 are iron sag inhibitors. MBOAT1/2 can inhibit iron sag by reshaping the phospholipid profile of cells, and their iron sag monitoring function is independent of GPX4 or FSP1. MBOAT1 and MBOAT2 are up-regulated by sex hormone receptors, namely estrogen receptor (ER) and androgen receptor (AR), respectively (PMID: 37267948). The calculated molecular weight of MBOAT1 is 57 kDa. This antibody can recognize the 40 kDa isoform of MBOAT1.