Validation Data Gallery
|Positive WB detected in
|fetal human brain tissue, human brain tissue, L02 cells, mouse liver tissue
|Positive IP detected in
|Positive IHC detected in
|human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11525-1-AP targets MCC in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|MCC fusion protein Ag2089
|mutated in colorectal cancers
|Calculated molecular weight
|829 aa, 93 kDa
|Observed molecular weight
|98 kDa, 113-118 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,
|Product Specific Protocols
|WB protocol for MCC antibody 11525-1-AP
|IHC protocol for MCC antibody 11525-1-AP
|IF protocol for MCC antibody 11525-1-AP
|IP protocol for MCC antibody 11525-1-AP
|Click here to view our Standard Protocols
'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.
Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.
Front Med (Lausanne)
MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.
Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.