Validation Data Gallery
|Positive WB detected in||fetal human brain tissue, human brain tissue, L02 cells, mouse liver tissue|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11525-1-AP targets MCC in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MCC fusion protein Ag2089|
|Full Name||mutated in colorectal cancers|
|Calculated molecular weight||829 aa, 93 kDa|
|Observed molecular weight||98 kDa, 113-118 kDa|
|GenBank accession number||BC018919|
|Gene ID (NCBI)||4163|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,
|Product Specific Protocols|
|WB protocol for MCC antibody 11525-1-AP||Download protocol|
|IHC protocol for MCC antibody 11525-1-AP||Download protocol|
|IF protocol for MCC antibody 11525-1-AP||Download protocol|
|IP protocol for MCC antibody 11525-1-AP||Download protocol|
|Click here to view our Standard Protocols|
'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.
Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.
Front Med (Lausanne)
MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.
Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.