MCC Polyclonal antibody

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  WB analysis of fetal human brain using 11525-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11525-1-AP

  WB result of MCC antibody (11525-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCC transfected HeLa cells.

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  WB analysis of L02 using 11525-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11525-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11525-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11525-1-AP

  IP result of anti-MCC (IP:11525-1-AP, 4ug; Detection:11525-1-AP 1:5000) with HeLa cells lysate 1280 ug.

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  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11525-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCC antibody (11525-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

MCC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11525-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MCC1

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Tested Applications

• Positive WB detected in: fetal human brain tissue, HeLa cells, human brain tissue, L02 cells, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

11525-1-AP targets MCC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2089

  Product name: Recombinant human MCC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 480-829 aa of BC018919

  Sequence: AGVGSSPGDQSGDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMAELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADAASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL

• Full Name: mutated in colorectal cancers
• Calculated Molecular Weight: 829 aa, 93 kDa
• Observed Molecular Weight: 98 kDa, 113-118 kDa
• GenBank Accession Number: BC018919
• Gene Symbol: MCC
• Gene ID (NCBI): 4163
• RRID: AB_2143796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23508
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Oncogene; 'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.; Authors - F A Benthani
human	WB	Proteomics; Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.; Authors - Corbo Claudia C
human,mouse	WB,IHC,IF	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song ; KD Validated
mouse	IHC	PLoS One.; Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.; Authors - Rohrbeck A
human	WB	bioRxiv; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan
human	WB	Nucleic Acids Res; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan