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MCL1 Monoclonal antibody

MCL1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat no : 66026-1-Ig

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM



Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, Jurkat cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66026-1-Ig is MCL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10609
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350 aa, 37 kDa
GenBank accession numberBC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

Protocols

Product Specific Protocols
WB protocol for MCL1 antibody 66026-1-IgDownload protocol
IHC protocol for MCL1 antibody 66026-1-IgDownload protocol
IF protocol for MCL1 antibody 66026-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Epigenetics

Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.

Authors - Yingyan Han
humanWB

J Nat Prod

Hernandezine Regulates Proliferation and Autophagy-Induced Apoptosis in Melanoma Cells.

Authors - Xiaoting Wang
humanWB

Mol Microbiol

The emerging oral pathogen, Filifactor alocis, extends the functional lifespan of human neutrophils.

Authors - Irina Miralda
humanWB

Ann Transl Med

Adipose-derived stem cells (ADSCs) inhibit the expression of anti-apoptosis proteins through up-regulation of ATF4 on breast cancer cells.

Authors - Jing Ren
mouse,ratWB

Endocrine

miR-25 and miR-92b regulate insulin biosynthesis and pancreatic β-cell apoptosis.

Authors - Zhiyi Shen
humanWB

Placenta

Downregulation of miR-29a/b/c in placenta accreta inhibits apoptosis of implantation site intermediate trophoblast cells by targeting MCL1.

Authors - Yongzhong Gu