MCL1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MCL1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E3C2

Cat no : 66026-1-PBS

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

---

Validation Data Gallery

• 

  WB analysis using 66026-1-Ig (same clone as 66026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

  WB result of MCL1 antibody (66026-1-Ig, 1:1000) with si-Control and si-MCL1 transfected Jurkat cells. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66026-1-Ig (same clone as 66026-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 66026-1-Ig (same clone as 66026-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66026-1-Ig (same clone as 66026-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66026-1-Ig (same clone as 66026-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66026-1-Ig (same clone as 66026-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66026-1-Ig (same clone as 66026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66026-1-Ig (MCL1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCL1 antibody (66026-1-Ig, Clone: 1E3C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66026-1-Ig (same clone as 66026-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66026-1-Ig (MCL1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66026-1-Ig (same clone as 66026-1-PBS)

  Immunofluorescent analysis of Hela cells, using MCL1 antibody 66026-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66026-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66026-1-PBS targets MCL1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MCL1 fusion protein Ag10609
• Full Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Calculated Molecular Weight: 350 aa, 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC107735
• Gene Symbol: MCL1
• Gene ID (NCBI): 4170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.