Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, mouse ovary tissue, mouse thymus tissue, mouse spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung squamous cell carcinoma tissue, human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13347-2-AP targets MCM6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4180 Product name: Recombinant human MCM6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 471-821 aa of BC032374 Sequence: VAIHEAMEQQTISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKHLRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEYCRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED Predict reactive species |
| Full Name | minichromosome maintenance complex component 6 |
| Calculated Molecular Weight | 821 aa, 93 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC032374 |
| Gene Symbol | MCM6 |
| Gene ID (NCBI) | 4175 |
| RRID | AB_2142537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MCM6 antibody 13347-2-AP | Download protocol |
| IHC protocol for MCM6 antibody 13347-2-AP | Download protocol |
| IP protocol for MCM6 antibody 13347-2-AP | Download protocol |
| WB protocol for MCM6 antibody 13347-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MCM6 Polyclonal antibody (Cat# 13347-2-AP) has 35 total citations published in journals including Nature Communications, Nature Cancer, Acta Pharmaceutica Sinica B, Journal of Hematology & Oncology, Advanced Science, Biomaterials, EMBO Molecular Medicine, Cell Proliferation, Oncotarget, eLife, Journal of Experimental & Clinical Cancer Research, Science China Life Sciences, and others. Associated research fields include DNA replication and licensing, cell cycle control, chromatin biology, angiogenesis, and multiple cancer types such as pancreatic, breast, lung, liver, bladder, glioma, gastric, and head and neck cancers.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma | ||
Nat Cancer Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. | ||
Nat Commun CRL4(DCAF12) regulation of MCMBP ensures optimal licensing of DNA replication. | ||
Acta Pharm Sin B Beyond ribosomal function: RPS6 deficiency suppresses cholangiocarcinoma cell growth by disrupting alternative splicing | ||
J Exp Clin Cancer Res MCM6 promotes metastasis of hepatocellular carcinoma via MEK/ERK pathway and serves as a novel serum biomarker for early recurrence. | ||
Adv Sci (Weinh) Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage |
