MCM6 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 13347-2-AP

Synonyms

P105MCM, Mis5, MCG40308, EC:3.6.4.12

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Validation Data Gallery

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  WB analysis using 13347-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13347-2-AP

  WB result of MCM6 antibody (13347-2-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

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  WB analysis of mouse ovary using 13347-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13347-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13347-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13347-2-AP

  IP result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.

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  IHC staining of human lung squamous cell carcinoma using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13347-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM6 antibody (13347-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, mouse ovary tissue, mouse thymus tissue, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung squamous cell carcinoma tissue, human cervical cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Product Information

13347-2-AP targets MCM6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCM6 fusion protein Ag4180
• Full Name: minichromosome maintenance complex component 6
• Calculated Molecular Weight: 821 aa, 93 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC032374
• Gene Symbol: MCM6
• Gene ID (NCBI): 4175
• RRID: AB_2142537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	Front Oncol; METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.; Authors - Dong-Dong Yang
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li