MCM7 Polyclonal antibody

MCM7 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 11225-1-AP

Synonyms

P1.1 MCM3, MCM2, EC:3.6.4.12, CDC47 homolog, CDC47

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 11225-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11225-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP (MCM7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11225-1-AP

  IP result of anti-MCM7 (IP:11225-1-AP, 4ug; Detection:11225-1-AP 1:2000) with NIH/3T3 cells lysate 1600ug.

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  IHC staining of human lung squamous cell carcinoma using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 11225-1-AP (MCM7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:50.

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  IHC staining of human lymphoma using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11225-1-AP (MCM7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 11225-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11225-1-AP (MCM7 Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 11225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MCM7 antibody (11225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11225-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MCM7 (11225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human lung squamous cell carcinoma tissue, human lung cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below

Product Information

11225-1-AP targets MCM7 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCM7 fusion protein Ag1730
• Full Name: minichromosome maintenance complex component 7
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013375
• Gene Symbol: MCM7
• Gene ID (NCBI): 4176
• RRID: AB_2297584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer; Authors - Huaiyuan Liang
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Elife; The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP; Authors - Maria Körner
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao