• Featured Product
  • KD/KO Validated

Midkine Polyclonal antibody

Midkine Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 11009-1-AP


ARAP, MDK, Midkine, MK, MK1, NEGF2

Tested Applications

Positive WB detected inmouse embryo tissue, COLO 320 cells, SH-SY5Y cells
Positive IP detected inmouse embryo tissue
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11009-1-AP targets Midkine in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Midkine fusion protein Ag1465
Full Name midkine (neurite growth-promoting factor 2)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC011704
Gene Symbol MDK
Gene ID (NCBI) 4192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.


Product Specific Protocols
WB protocol for Midkine antibody 11009-1-APDownload protocol
IHC protocol for Midkine antibody 11009-1-APDownload protocol
IF protocol for Midkine antibody 11009-1-APDownload protocol
IP protocol for Midkine antibody 11009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani

Front Immunol

Roles of the m6A Modification of RNA in the Glioblastoma Microenvironment as Revealed by Single-Cell Analyses.

Authors - Feng Yuan
  • KD Validated

Front Oncol

Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins.

Authors - Luyu Zheng

Cancer Cell Int

Midkine promotes glioblastoma progression via PI3K-Akt signaling.

Authors - Beiquan Hu
  • KD Validated

Am J Respir Cell Mol Biol

Mesenchymal Glucocorticoid Receptor Regulates Development of Multiple Cell Layers of the Mouse Lung.

Authors - Bird A Daniel AD

J Mol Neurosci

Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.

Authors - Ying Xing