MEN1 Monoclonal antibody

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  WB analysis using 68473-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68473-1-Ig (MEN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 68473-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68473-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68473-1-Ig (MEN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 68473-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.2 μg MEN1 Monoclonal antibody (68473-1-Ig, Clone:3A6A11) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005)(N/A), or 0.2 μg KLH (66360-1-Ig, Clone: T1F8D3F10) (N/A). Cells were fixed with 4% PFA .

MEN1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68473-1-Ig

Clone No.3A6A11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

3A6A11, MEAI, Menin, Menin 1, SCG2

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, U2OS cells, LNCaP cells, K-562 cells, Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68473-1-Ig targets MEN1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7566

  Product name: Recombinant human MEN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 226-575 aa of BC002544

  Sequence: QKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPAASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL

• Full Name: multiple endocrine neoplasia I
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC002544
• Gene Symbol: MEN1
• Gene ID (NCBI): 4221
• RRID: AB_3085184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00255
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. It is located in nucleus and ubiquitous expression in spleen and lymph node. The calculated molecular weight of MEN1 is 68 kDa. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice.