METAP1 Polyclonal antibody, PBS Only

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  WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 28702-1-AP (same clone as 28702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28702-1-AP (METAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 28702-1-AP (same clone as 28702-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using METAP1 antibody (28702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

METAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28702-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Peptidase M 1, MetAP 1, MAP 1, KIAA0094

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Product Information

28702-1-PBS targets METAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METAP1 fusion protein Ag29828
• Full Name: methionyl aminopeptidase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: NM_015143
• Gene Symbol: METAP1
• Gene ID (NCBI): 23173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53582
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

METAP1 (methionyl aminopeptidase 1), also known as MAP1A or MetAP1A, is expected to be located in cytosol and widely expressed in esophagus and testis. The calculated molecular weight of METAP1 is 43 kDa. METAP1 is expected to have aminopeptidase activity and metallo-exopeptidase activity. It is also involved in platelet aggregation. At the same time, HsMetAP1 participates in co-translation to remove N- terminal methionine from newborn protein. Studies have shown that when HsMetAP1 is knocked down by gene-specific siRNA, cells show slow progress in G(2)/M phase, which is similar to cells treated with MetAP1 inhibitors. Importantly, MetAP1 inhibitors can induce apoptosis of leukemia cell lines, which may be the result of their interference with G(2)/M checkpoint. Therefore, it is necessary for normal cell cycle, especially in G(2)/M phase of cell cycle, and it can be used as a promising target for discovering and developing new anticancer drugs (PMID: 17114291).