MFSD2 Polyclonal antibody

MFSD2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30555-1-AP

Synonyms

FLJ14490, FLJ35904, FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341

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Validation Data Gallery

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  WB analysis using 30555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30555-1-AP (MFSD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 30555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30555-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 30555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 30555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30555-1-AP targets MFSD2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFSD2 fusion protein Ag33064
• Full Name: major facilitator superfamily domain containing 2
• Calculated Molecular Weight: 543 aa, 60 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC092414
• Gene Symbol: MFSD2
• Gene ID (NCBI): 84879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFSD2 is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. MFSD2 is involved in the establishment of the blood-brain barrier and is required for brain growth and function.

Publications

Species	Application	Title
mouse	WB	Bioeng Transl Med; Sound conditioning strategy promoting paracellular permeability of the blood-labyrinth-barrier benefits inner ear drug delivery; Authors - Xueling Wang