Tested Applications
| Positive WB detected in | Ramos cells, Raji cells |
| Positive IHC detected in | human gliomas tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11454-1-AP targets MZB1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2015 Product name: Recombinant human MZB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC021275 Sequence: MRLSLPLLLLLLGAWAIPGGLGDRAPLTATAPQLDDEEMYSAHMPAHLRCDACRAVAYQMWQNLAKAETKLHTSNSGGRRELSELVYTDVLDRSCSRNWQDYGVREVDQVKRLTGPGLSEGPEPSISVMVTGGPWPTRLSRTCLHYLGEFGEDQIYEAHQQGRGALEALLCGGPQGACSEKVSATREEL Predict reactive species |
| Full Name | hypothetical protein MGC29506 |
| Calculated Molecular Weight | 189 aa, 21 kDa |
| Observed Molecular Weight | 19-21 kDa |
| GenBank Accession Number | BC021275 |
| Gene Symbol | MZB1 |
| Gene ID (NCBI) | 51237 |
| RRID | AB_832820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WU39 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MZB1 antibody 11454-1-AP | Download protocol |
| WB protocol for MZB1 antibody 11454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 11454-1-AP (anti-MZB1) has 10 total citations published in journals including Advanced Science, Clinical Cancer Research, Journal of Pathology, Arthritis Research & Therapy, International Journal of Cancer, Anais Brasileiros de Dermatologia, Journal of Biological Chemistry, Oncology Letters, Open Medicine, and Journal of Surgical Research. Research fields span lung adenocarcinoma, hepatocellular carcinoma, renal carcinoma, systemic lupus erythematosus, gastric cancer, leprosy, T-cell development, breast cancer, ovarian cancer, and pancreatic cancer.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) SLPI⁺ AT2-Like Cells Orchestrate Lung Adenocarcinoma Invasion via Wnt Pathway Activation and Stromal Crosstalk in a Spatially Defined Margin Niche. | ||
Clin Cancer Res Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma. | ||
J Pathol Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney. | ||
Arthritis Res Ther Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes. | ||
Int J Cancer Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer. | ||
