MGST2 Polyclonal antibody

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  IP experiment of HUVEC using 25451-1-AP

  IP result of anti-MGST2 (IP:25451-1-AP, 4ug; Detection:25451-1-AP 1:400) with HUVEC cells lysate 1040 ug.

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  IP experiment of HuH-7 using 25451-1-AP

  IP result of anti-MGST2 (IP:25451-1-AP, 4ug; Detection:25451-1-AP 1:400) with HuH-7 cells lysate 1040 ug.

MGST2 Polyclonal Antibody for IP, ELISA

Cat No. 25451-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

Microsomal GST II, Microsomal GST 2, MGST II, GST2

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Tested Applications

• Positive IP detected in: HuH-7 cells, HUVEC cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25451-1-AP targets MGST2 in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21105

  Product name: Recombinant human MGST2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-147 aa of BC025416

  Sequence: MAGNSILLAAVSILSACQQSYFALQVGKARLKYKVTPPAVTGSPEFERVFRAQQNCVEFYPIFIITLWMAGWYFNQVFATCLGLVYIYGRHLYFWGYSEAAKKRITGFRLSLGILALLTLLGALGIANSFLDEYLDLNIAKKLRRQF

• Full Name: microsomal glutathione S-transferase 2
• Calculated Molecular Weight: 147 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC025416
• Gene Symbol: MGST2
• Gene ID (NCBI): 4258
• RRID: AB_3669476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99735
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal glutathione S-transferase 2 (MGST2) is a 17 kDa trimeric integral membrane protein homologous to leukotriene C4 synthase (LTC4S) and catalyze the biosynthesis of the proinflammatory mediator leukotriene C4 (LTC4) in cells devoid of LTC4S (PMID: 23409838).