MICA/MICB Recombinant monoclonal antibody

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  WB analysis using 83725-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83725-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

MICA/MICB Recombinant Antibody for WB, ELISA

Cat No. 83725-5-RR

Clone No.240735D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D9

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: MHC class I polypeptide-related sequence A
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: AAH16929.1
• Gene Symbol: MICA
• Gene ID (NCBI): 100507436
• RRID: AB_3671325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q29983
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.