MICA/MICB Recombinant monoclonal antibody

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• MICA/MICB Recombinant monoclonal antibody (83725-5-RR)

MICA/MICB Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83725-5-RR Copied!

Clone No. 240735D9

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D9

• 

  WB analysis using 83725-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83725-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: MHC class I polypeptide-related sequence A
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: /
• Gene Symbol: MICA
• Gene ID (NCBI): 100507436
• RRID: AB_3671325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q29983
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

Publications

What published studies show

The MICA/MICB Recombinant monoclonal antibody (240735D9), catalog number 83725-5-RR, has 1 citation. It appears in Cell Reports in Medicine, in a study on NKG2D overexpression potentiating CAR-T cells through costimulation by tumor NKG2D ligands. The associated research field is immunotherapy, specifically CAR-T cell therapy and NK cell–mediated immunity involving MICA/MICB–NKG2D interactions.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell Rep Med; NKG2D overexpression potentiates CAR-T cells through costimulation by tumor NKG2D ligands.; Authors - Jiajia Li