Tested Applications
| Positive WB detected in | DU 145 cells, PC-3 cells, U-251 cells, U-87 MG cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13965-1-AP targets MICAL2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4950 Product name: Recombinant human MICAL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 771-1124 aa of BC044577 Sequence: KAAHLASMFGHGDFPQNKLLSKGLSHTHPPSPPSRLPSPDPAASSSPSTVDSASPARKEKKSPSGFHFHPSHLRTVHPQLTVGKVSSGIGAAAEVLVNLYMNDHRPKAQATSPDLESMRKSFPLNLGGSDTCYFCKKRVYVMERLSAEGHFFHRECFRCSICATTLRLAAYTFDCDEGKFYCKPHFIHCKTNSKQRKRRAELKQQREEEATWQEQEAPRRDTPTESSCAVAAIGTLEGSPPVHFSLPVLHPLLG Predict reactive species |
| Full Name | microtubule associated monoxygenase, calponin and LIM domain containing 2 |
| Calculated Molecular Weight | 127 kDa |
| Observed Molecular Weight | 95 kDa, 112 kDa |
| GenBank Accession Number | BC044577 |
| Gene Symbol | MICAL2 |
| Gene ID (NCBI) | 9645 |
| RRID | AB_2143764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MICAL2 antibody 13965-1-AP | Download protocol |
| IHC protocol for MICAL2 antibody 13965-1-AP | Download protocol |
| WB protocol for MICAL2 antibody 13965-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MICAL2 Antibody (Cat# 13965-1-AP) has 13 citations published in journals including J Nanobiotechnology, Theranostics, Cell Death Dis, Cancer Lett, J Transl Med, Oncogene, Acta Physiol, Oxid Med Cell Longev, Front Mol Biosci, Prog Neuropsychopharmacol Biol Psychiatry, Microvasc Res, Horm Cancer, and J Appl Genet. Research fields span multiple cancers (lung, breast, gastric, cervical, head and neck, pancreatic, nasopharyngeal, endometrial), skin aging, renal tubular dysfunction, pulmonary vascular disease, and depression-related NAFLD.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging | ||
Theranostics Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction. | ||
Cancer Lett MICAL2 is a novel nucleocytoplasmic shuttling protein promoting cancer invasion and growth of lung adenocarcinoma. | ||
J Transl Med Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer
| ||
Oncogene Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement
|
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user tested it on H4 cell lysate run on an SDS-PAGE gel, using a 1:500 dilution with aMICAL2 in 3% BSA (TBS-Tween 0.1%). The result was positive, indicating the antibody performed well for detecting the target in this cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kincaid (Verified Customer) (06-02-2024) | H4 cell lysate run on SDS-PAGE gel. aMICAL2 diluted in 3% BSA, in TBS-tween (0.1%).
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