MIG Polyclonal antibody

MIG Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22355-1-AP

Synonyms

C X C motif chemokine 9, CMK, crg 10, CXCL9, Humig, MIG, MIG; CXCL9, SCYB9, Small inducible cytokine B9

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Validation Data Gallery

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  IHC staining of human colon cancer using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22355-1-AP (MIG antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22355-1-AP (MIG antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 22355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22355-1-AP (MIG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below

Product Information

The immunogen of 22355-1-AP is MIG Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIG fusion protein Ag17932
• Full Name: chemokine (C-X-C motif) ligand 9
• Calculated molecular weight: 14 kDa
• GenBank accession number: BC095396
• Gene symbol: CXCL9
• Gene ID (NCBI): 4283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.

Publications

Species	Application	Title
human	IHC	Front Immunol; Avoiding Absolute Quantification Trap: A Novel Predictive Signature of Clinical Benefit to Anti-PD-1 Immunotherapy in Non-Small Cell Lung Cancer.; Authors - Chengming Liu
human	IHC	Cancer Cell Int; Activation of the chemokine receptor 3 pathway leads to a better response to immune checkpoint inhibitors in patients with metastatic urothelial carcinoma.; Authors - Wenqin Feng
human	IHC	J Plast Surg Hand Surg; A comparison expression analysis of CXCR4, CXCL9 and Caspase-9 in dermal vascular endothelial cells between keloids and normal skin on chemotaxis and apoptosis.; Authors - Xinhang Dong
human	WB,IHC	Cancer Immunol Immunother; CXCL9 influences the tumor immune microenvironment by stimulating JAK/STAT pathway in triple-negative breast cancer; Authors - Lei Wu