MITF Polyclonal antibody

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  WB analysis using 13092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 13092-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13092-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13092-1-AP

  IP result of anti-MITF (IP:13092-1-AP, 4ug; Detection:13092-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  FC experiment of HeLa using 13092-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MITF Polyclonal antibody (13092-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MITF Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Cat No. 13092-1-AP

Publications(36)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC (Intra), IP, ELISA

Synonyms

WS2A, Microphthalmia-associated transcription factor, MI, Class E basic helix-loop-helix protein 32, bHLHe32

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, mouse heart tissue, rat skin tissue, NIH/3T3 cells
• Positive IP detected in: mouse heart tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 31 publications below
• IF: See 5 publications below

Product Information

13092-1-AP targets MITF in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MITF fusion protein Ag3679
• Full Name: microphthalmia-associated transcription factor
• Calculated Molecular Weight: 91 aa, 10 kDa, 59 kDa
• Observed Molecular Weight: 59-65 kDa
• GenBank Accession Number: BC012503
• Gene Symbol: MITF
• Gene ID (NCBI): 4286
• RRID: AB_10597698
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75030
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

Publications

Species	Application	Title
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB	J Clin Invest; mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.; Authors - Kaushal Asrani
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
human,mouse	WB	Phytomedicine; Taxifolin inhibits melanoma proliferation/migration impeding USP18/Rac1/JNK/β-catenin oncogenic signaling; Authors - Libo Xu
human, mouse	WB	J Invest Dermatol; PMID: 23223141; Authors - Lee Chang Seok CS
human	WB	Pigment Cell Melanoma Res; D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.; Authors - Jisu Park

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eva (Verified Customer) (07-01-2025)

The antibody is functional both with and without antigen retrieval (AR: 110ºC for 1 min, a second cycle of 90ºC for 10s, in a citrate buffer 10mM pH=6); however, signal specificity appears reduced when antigen retrieval is applied, and its use is therefore not recommended. After x3 PBST (PBS + 0,2% Tween) washes, cryostat sample slides were incubated 2 hours with blocking buffer (PBST 0,2% + 5% FBS + 1% BSA) at Room Temperature (RT). The primary antibody was diluted 1:500 in Blocking Buffer and incubated with the samples overnight at 4 °C. Next day, wash x3 with PBST 0,2%, and incubate second antibody with fluorocrome at 1:500 2-3 hours at RT. Some background staining is observed, likely due to a high concentration of the secondary antibody, as it does not correspond to a specific signal. The staining is specifically localized in nuclei of pigmented cells, as expected, and no nonspecific signal is detected in other tissue types within the sample.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: iPS cell line differentiated into Retinal Organoids with Retinal Pigmented Epithelia Tissue

MITF Antibody Immunofluorescence validation (1:500 dilution) in iPS cell line differentiated into Retinal Organoids with Retinal Pigmented Epithelia Tissue (Cat no:13092-1-AP)

FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence