MITF Polyclonal antibody

MITF Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC,ELISA

Conjugate

Unconjugated

Cat no : 13092-1-AP

Synonyms

bHLHe32, MI, MITF, WS2A



Tested Applications

Positive WB detected inrat skin tissue, HeLa cells, Jurkat cells, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive FC detected inhESC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

13092-1-AP targets MITF in WB, IP, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MITF fusion protein Ag3679
Full Name microphthalmia-associated transcription factor
Calculated molecular weight 91 aa, 10 kDa, 59 kDa
Observed molecular weight 59-65 kDa
GenBank accession numberBC012503
Gene symbol MITF
Gene ID (NCBI) 4286
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID:22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

Protocols

Product Specific Protocols
WB protocol for MITF antibody 13092-1-APDownload protocol
IP protocol for MITF antibody 13092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biol Pharm Bull

Coumestrol Down-Regulates Melanin Production in Melan-a Murine Melanocytes through Degradation of Tyrosinase.

Authors - Jeong Ah Hwang
mouseWB

Arch Pharm Res

Triterpene glycosides with stimulatory activity on melanogenesis from the aerial parts of Weigela subsessilis.

Authors - Yu-Mi Won
humanWB

PLoS One

Ultraviolet B irradiation enhances the secretion of exosomes by human primary melanocytes and changes their exosomal miRNA profile.

Authors - Zeren Shen
mouseWB

Arch Pharm Res

Phenylacylphenol derivatives with anti-melanogenic activity from Stewartia pseudocamellia.

Authors - Zuh-Kyung Seong
mouseWB

PLoS One

(E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression.

Authors - Jisu Park
humanWB

Sci Rep

D-tyrosine adds an anti-melanogenic effect to cosmetic peptides.

Authors - Jisu Park