MITF Polyclonal antibody

MITF Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 13092-1-AP

Synonyms

bHLHe32, MI, MITF, WS2A

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Validation Data Gallery

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  WB analysis of rat skin using 13092-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13092-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13092-1-AP

  IP Result of anti-MITF (IP:13092-1-AP, 4ug; Detection:13092-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  FC experiment of hESC cells using 13092-1-AP

  FC result of anti-MITF (13092-1-AP, 1:50) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Red MITF).

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Tested Applications

• Positive WB detected in: rat skin tissue, mouse heart tissue, Jurkat cells
• Positive IP detected in: mouse heart tissue
• Positive FC detected in: hESC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 19 publications below
• IF: See 2 publications below

Product Information

13092-1-AP targets MITF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MITF fusion protein Ag3679
• Full Name: microphthalmia-associated transcription factor
• Calculated molecular weight: 91 aa, 10 kDa, 59 kDa
• Observed molecular weight: 59-65 kDa
• GenBank accession number: BC012503
• Gene symbol: MITF
• Gene ID (NCBI): 4286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

Publications

Species	Application	Title
mouse	WB	J Clin Invest; mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.; Authors - Kaushal Asrani
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
human, mouse	WB	J Invest Dermatol; PMID: 23223141; Authors - Lee Chang Seok CS
human	WB	Pigment Cell Melanoma Res; D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.; Authors - Jisu Park
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	Int J Mol Sci; Glucose Exerts an Anti-Melanogenic Effect by Indirect Inactivation of Tyrosinase in Melanocytes and a Human Skin Equivalent.; Authors - Sung Hoon Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence