MLL4 Polyclonal antibody

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  WB analysis using 31396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31396-1-AP (MLL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 31396-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MLL4 antibody (31396-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

MLL4 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31396-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MLL1B, KMT2B, KIAA0304, HRX2, DYT28

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31396-1-AP targets MLL4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35385

  Product name: Recombinant human MLL4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 800-982 aa of BC009337

  Sequence: KVQLFKIDQQQQQKVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESPVQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDLATEDTSSASETESVPSRSRRGKVEAAGPGGESEPTGSGGTLAHTPRRSLPSHHGKKMRMARCGHCRGCLRVQDCGSCV

• Full Name: myeloid/lymphoid or mixed-lineage leukemia 4
• Observed Molecular Weight: 180kDa;320kDa
• GenBank Accession Number: BC009337
• Gene Symbol: MLL4
• Gene ID (NCBI): 9757
• RRID: AB_3669963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UMN6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating 320 kDa N-terminal (MLL-N) and 180 kDa C-terminal (MLL-C) fragments (PMID: 24213472).