Melanophilin Monoclonal antibody
Melanophilin Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1A8D12
Cat no : 66092-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, pig brain tissue, PC-3 cells |
Positive IHC detected in | human kidney tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 66092-1-Ig is Melanophilin Fusion Protein expressed in E. coli.
Tested Reactivity | human, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Melanophilin fusion protein Ag19286 |
Full Name | melanophilin |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC001653 |
Gene symbol | MLPH |
Gene ID (NCBI) | 79083 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
Protocols
Product Specific Protocols | |
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WB protocol for Melanophilin antibody 66092-1-Ig | Download protocol |
IHC protocol for Melanophilin antibody 66092-1-Ig | Download protocol |
IF protocol for Melanophilin antibody 66092-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Med Rep Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes. |