Melanophilin Monoclonal antibody

Melanophilin Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A8D12

Cat no : 66092-1-Ig

Synonyms

Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a



Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, PC-3 cells
Positive IHC detected inhuman kidney tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66092-1-Ig is Melanophilin Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Melanophilin fusion protein Ag19286
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Protocols

Product Specific Protocols
WB protocol for Melanophilin antibody 66092-1-IgDownload protocol
IHC protocol for Melanophilin antibody 66092-1-IgDownload protocol
IF protocol for Melanophilin antibody 66092-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes.

Authors - Shuhui Qi