Filter: AllWBIHCIF/ICC

WB analysis using 66092-1-Ig (same clone as 66092-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66092-1-Ig (same clone as 66092-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66092-1-Ig (same clone as 66092-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 66092-1-Ig (same clone as 66092-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 66092-1-Ig (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66092-1-Ig (Melanophilin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66092-1-Ig (same clone as 66092-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney slide using 66092-1-Ig (Melanophilin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66092-1-Ig (same clone as 66092-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66092-1-Ig (Melanophilin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66092-1-PBS in a different storage buffer formulation.

MLPH Monoclonal antibody, PBS Only

MLPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66092-1-PBS

Clone No.1A8D12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A, SLAC2A

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66092-1-PBS targets MLPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag19286 Product name: Recombinant human MLPH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 8-236 aa of BC001653 Sequence: SKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKA Predict reactive species
Full Name	melanophilin
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC001653
Gene Symbol	MLPH
Gene ID (NCBI)	79083
RRID	AB_11232039
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BV36
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.