CoraLite®594-conjugated Melanophilin Monoclonal antibody

Melanophilin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A8D12

Cat no : CL594-66092

Synonyms

Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66092-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66092 is Melanophilin Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Melanophilin fusion protein Ag19286
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Protocols

Product Specific Protocols
IF protocol for CL594 Melanophilin antibody CL594-66092Download protocol
Standard Protocols
Click here to view our Standard Protocols