Validation Data Gallery
|Positive WB detected in||K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human skeletal muscle tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13614-1-AP targets MLXIP in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MLXIP fusion protein Ag4519|
|Full Name||MLX interacting protein|
|Calculated molecular weight||919 aa, 100 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC039704|
|Gene ID (NCBI)||22877|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).
|Product Specific Protocols|
|WB protocol for MLXIP antibody 13614-1-AP||Download protocol|
|IHC protocol for MLXIP antibody 13614-1-AP||Download protocol|
|IF protocol for MLXIP antibody 13614-1-AP||Download protocol|
|IP protocol for MLXIP antibody 13614-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.
MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.
Proc Natl Acad Sci U S A
Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.
MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.
Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.