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  • KD/KO Validated

MLXIP Polyclonal antibody

MLXIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13614-1-AP


bHLHe36, KIAA0867, MIR, MLX interacting protein, MLXIP, MONDOA

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13614-1-AP targets MLXIP in WB, IP, IHC, IF, chIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLXIP fusion protein Ag4519
Full Name MLX interacting protein
Calculated molecular weight 919 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC039704
Gene symbol MLXIP
Gene ID (NCBI) 22877
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).


Product Specific Protocols
WB protocol for MLXIP antibody 13614-1-APDownload protocol
IHC protocol for MLXIP antibody 13614-1-APDownload protocol
IF protocol for MLXIP antibody 13614-1-APDownload protocol
IP protocol for MLXIP antibody 13614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Cellular acidosis triggers human MondoA transcriptional activity by driving mitochondrial ATP production.

Authors - Blake R Wilde

JCI Insight

PMID: 31287806

Authors - Byungyong Ahn

Antioxid Redox Signal

Dl-3-n-Butylphthalide Inhibits NLRP3 Inflammasome and Mitigates Alzheimer's-Like Pathology via Nrf2-TXNIP-TrX Axis.

Authors - Chun-Yan Wang

Diabetes Metab J

MondoA Is Required for Normal Myogenesis and Regulation of the Skeletal Muscle Glycogen Content in Mice.

Authors - Hui Ran
  • KD Validated

Mol Cell Biol

Ras Suppresses TXNIP Expression by Restricting Ribosome Translocation.

Authors - Zhizhou Ye

Cancer Metab

Protein synthesis inhibitors stimulate MondoA transcriptional activity by driving an accumulation of glucose 6-phosphate.

Authors - Blake R Wilde
  • KO Validated