MLXIP Polyclonal antibody

• Featured Product
• KD/KO Validated

MLXIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 13614-1-AP

Synonyms

MLX-interacting protein, MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36

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Validation Data Gallery

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  WB analysis of K-562 using 13614-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13614-1-AP (MLXIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13614-1-AP

  IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IP experiment of K-562 using 13614-1-AP

  IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13614-1-AP

  Immunofluorescent analysis of HepG2 cells, using MLXIP antibody 13614-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human skeletal muscle tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• ChIP: See 6 publications below

Product Information

13614-1-AP targets MLXIP in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLXIP fusion protein Ag4519
• Full Name: MLX interacting protein
• Calculated Molecular Weight: 919 aa, 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC039704
• Gene Symbol: MLXIP
• Gene ID (NCBI): 22877
• RRID: AB_2282153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Publications

Species	Application	Title
human	WB	Cancer Cell; Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.; Authors - Patrick A Carroll
mouse	chIP	Nat Neurosci; PMID: 31636448; Authors - Hei-Man Chow
mouse	WB,FC,ChIP	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	ChIP	Proc Natl Acad Sci U S A; Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.; Authors - Liangliang Shen
human	WB	Diabetes; MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.; Authors - Paul Richards
human	WB	PLoS Pathog; Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.; Authors - Erica L Sanchez