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MLXIP Polyclonal antibody

MLXIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13614-1-AP

Synonyms

MLX-interacting protein, MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13614-1-AP targets MLXIP in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLXIP fusion protein Ag4519
Full Name MLX interacting protein
Calculated Molecular Weight 919 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039704
Gene Symbol MLXIP
Gene ID (NCBI) 22877
RRIDAB_2282153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Protocols

Product Specific Protocols
WB protocol for MLXIP antibody 13614-1-APDownload protocol
IHC protocol for MLXIP antibody 13614-1-APDownload protocol
IF protocol for MLXIP antibody 13614-1-APDownload protocol
IP protocol for MLXIP antibody 13614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.

Authors - Patrick A Carroll
mousechIP

Nat Neurosci

PMID: 31636448

Authors - Hei-Man Chow
mouseWB,FC,ChIP

Gastroenterology

MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.

Authors - Ying Lu
humanChIP

Proc Natl Acad Sci U S A

Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.

Authors - Liangliang Shen
humanWB

Diabetes

MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.

Authors - Paul Richards
humanWB

PLoS Pathog

Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.

Authors - Erica L Sanchez