Neprilysin/CD10 Polyclonal antibody
Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 18008-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, human kidney tissue, rat kidney tissue |
Positive IP detected in | Raji cells |
Positive IHC detected in | human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human kidney tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 15 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
Product Information
18008-1-AP targets Neprilysin/CD10 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Neprilysin/CD10 fusion protein Ag12506 |
Full Name | membrane metallo-endopeptidase |
Calculated Molecular Weight | 80aa,9 kDa; 750aa,85 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC106070 |
Gene Symbol | CD10 |
Gene ID (NCBI) | 4311 |
RRID | AB_2146541 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
Protocols
Product Specific Protocols | |
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WB protocol for Neprilysin/CD10 antibody 18008-1-AP | Download protocol |
IHC protocol for Neprilysin/CD10 antibody 18008-1-AP | Download protocol |
IF protocol for Neprilysin/CD10 antibody 18008-1-AP | Download protocol |
IP protocol for Neprilysin/CD10 antibody 18008-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression | ||
Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation. | ||
Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. | ||
Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. | ||
J Alzheimers Dis Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (10-04-2021) | calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.
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