MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18008-1-AP


Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Positive FC detected inRamos cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18008-1-AP targets MME,CD10 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag12506
Full Name membrane metallo-endopeptidase
Calculated Molecular Weight80aa,9 kDa; 750aa,85 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC106070
Gene Symbol MME
Gene ID (NCBI) 4311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).


Product Specific Protocols
WB protocol for MME,CD10 antibody 18008-1-APDownload protocol
IHC protocol for MME,CD10 antibody 18008-1-APDownload protocol
IF protocol for MME,CD10 antibody 18008-1-APDownload protocol
IP protocol for MME,CD10 antibody 18008-1-APDownload protocol
FC protocol for MME,CD10 antibody 18008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

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MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu

Int J Biol Macromol

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Authors - Hui Zeng

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

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BMC Genomics

Genetic control of renal tumorigenesis by the mouse Rtm1 locus.

Authors - Jensen José Ricardo JR

J Alzheimers Dis

Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.

Authors - Ling-Li Lv


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

  • Applications: Western Blot