MME,CD10 Polyclonal antibody
MME,CD10 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 18008-1-AP
Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, human kidney tissue, rat kidney tissue|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human kidney tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human kidney tissue|
|Positive FC detected in||Ramos cells, Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 11 publications below|
|IHC||See 3 publications below|
|IF||See 1 publications below|
18008-1-AP targets MME,CD10 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MME,CD10 fusion protein Ag12506|
|Full Name||membrane metallo-endopeptidase|
|Calculated molecular weight||80aa,9 kDa; 750aa,85 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC106070|
|Gene ID (NCBI)||4311|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
|Product Specific Protocols|
|WB protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IHC protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IF protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IP protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|FC protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|Click here to view our Standard Protocols|
MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.
Int J Biol Macromol
A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.
Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.
Genetic control of renal tumorigenesis by the mouse Rtm1 locus.
J Alzheimers Dis
Dendrobium nobile Lindl. Alkaloids Ameliorate Cognitive Dysfunction in Senescence Accelerated SAMP8 Mice by Decreasing Amyloid-β Aggregation and Enhancing Autophagy Activity.
J Alzheimers Dis
Ameliorating Ribosylation-Induced Amyloid-β Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (10-04-2021)
calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.