Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, human kidney tissue, rat kidney tissue|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human kidney tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human kidney tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18008-1-AP targets MME,CD10 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MME,CD10 fusion protein Ag12506|
|Full Name||membrane metallo-endopeptidase|
|Calculated molecular weight||80aa,9 kDa; 750aa,85 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC106070|
|Gene ID (NCBI)||4311|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).
|Product Specific Protocols|
|WB protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IHC protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IF protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|IP protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|FC protocol for MME,CD10 antibody 18008-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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