MMP13 Polyclonal antibody
MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
271
Cat no : 18165-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 201 publications below |
IHC | See 95 publications below |
IF | See 65 publications below |
Product Information
18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, pig, rabbit, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MMP13 fusion protein Ag12653 |
Full Name | matrix metallopeptidase 13 |
Calculated molecular weight | 471 aa, 54 kDa |
Observed molecular weight | 65-70 kDa, 50-55 kDa, 40 kDa |
GenBank accession number | BC074808 |
Gene symbol | MMP13 |
Gene ID (NCBI) | 4322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MMP13 antibody 18165-1-AP | Download protocol |
IHC protocol for MMP13 antibody 18165-1-AP | Download protocol |
IF protocol for MMP13 antibody 18165-1-AP | Download protocol |
IP protocol for MMP13 antibody 18165-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Mol Med SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis | ||
Nat Commun Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing. | ||
Nat Commun Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. | ||
Bone Res Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration. | ||
Bone Res FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xie (Verified Customer) (05-13-2021) | Good IHC staining for Rat MMP13.
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FH Hongxue (Verified Customer) (01-12-2021) | clear wb band
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