Validation Data Gallery
|Positive WB detected in
|HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue, HepG2 cells, MDA-MB-453s cells
|Positive IP detected in
|Positive IHC detected in
|human liver tissue, human hepatocirrhosis tissue, mouse liver tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, rabbit, zebrafish, pig, bovine
|Host / Isotype
|Rabbit / IgG
|MMP13 fusion protein Ag12653
|matrix metallopeptidase 13
|Calculated molecular weight
|471 aa, 54 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).
|Product Specific Protocols
|WB protocol for MMP13 antibody 18165-1-AP
|IHC protocol for MMP13 antibody 18165-1-AP
|IF protocol for MMP13 antibody 18165-1-AP
|IP protocol for MMP13 antibody 18165-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xie (Verified Customer) (05-13-2021)
Good IHC staining for Rat MMP13.
Hongxue (Verified Customer) (01-12-2021)
clear wb band