MMP13 Polyclonal antibody

MMP13 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18165-1-AP

Synonyms

CLG3, Matrix metalloproteinase 13, MMP 13, MMP13



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, MDA-MB-453s cells, zebrafish tissue, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rabbit, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP13 fusion protein Ag12653
Full Name matrix metallopeptidase 13
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 65-70 kDa, 50-55 kDa, 40 kDa
GenBank accession numberBC074808
Gene symbol MMP13
Gene ID (NCBI) 4322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Protocols

Product Specific Protocols
WB protocol for MMP13 antibody 18165-1-APDownload protocol
IHC protocol for MMP13 antibody 18165-1-APDownload protocol
IF protocol for MMP13 antibody 18165-1-APDownload protocol
IP protocol for MMP13 antibody 18165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Inflammation

Effects of IL-1β on MMP-9 Expression in Cementoblast-Derived Cell Line and MMP-Mediated Degradation of Type I Collagen.

Authors - Mingyuan Du
humanWB

Int J Oncol

25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway.

Authors - Saisai Wang
humanWB

EBioMedicine

Cortistatin binds to TNF-α receptors and protects against osteoarthritis.

Authors - Yunpeng Zhao
mouseIHC

J Cell Physiol

Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running.

Authors - Zilong Yao
humanWB

Bone

miR-93-5p attenuates IL-1β-induced chondrocyte apoptosis and cartilage degradation in osteoarthritis partially by targeting TCF4.

Authors - Huaming Xue
mouseIHC

Biomaterials

miR-100-5p-abundant exosomes derived from infrapatellar fat pad MSCs protect articular cartilage and ameliorate gait abnormalities via inhibition of mTOR in osteoarthritis.

Authors - Jiangyi Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xie (Verified Customer) (05-13-2021)

Good IHC staining for Rat MMP13.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: rat knee joints
MMP13 Antibody Immunohistochemistry validation (1:100 dilution) in rat knee joints (Cat no:18165-1-AP)
FH

Hongxue (Verified Customer) (01-12-2021)

clear wb band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
MMP13 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:18165-1-AP)