MMP13 Polyclonal antibody

MMP13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

271

Reviews (2)

Cat no : 18165-1-AP

Synonyms

CLG3, Matrix metalloproteinase 13, MMP 13, MMP13

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Validation Data Gallery

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  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 18165-1-AP

  IP result of anti-MMP13 (IP:18165-1-AP, 4ug; Detection:18165-1-AP 1:300) with MCF-7 cells lysate 2480 ug.

• 

  IHC staining of human breast cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 18165-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18165-1-AP (MMP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 18165-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, MDA-MB-453s cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 201 publications below
• IHC: See 95 publications below
• IF: See 65 publications below

Product Information

18165-1-AP targets MMP13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, pig, rabbit, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP13 fusion protein Ag12653
• Full Name: matrix metallopeptidase 13
• Calculated molecular weight: 471 aa, 54 kDa
• Observed molecular weight: 65-70 kDa, 50-55 kDa, 40 kDa
• GenBank accession number: BC074808
• Gene symbol: MMP13
• Gene ID (NCBI): 4322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

Publications

Species	Application	Title
human,mouse	IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse,rat	WB,IF	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao
mouse	IF	Nat Commun; Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing.; Authors - Kishor K Sivaraj
mouse	WB,IHC	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
human	IF	Bone Res; Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.; Authors - Dong Wang
human	IHC	Bone Res; FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.; Authors - Dong Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xie (Verified Customer) (05-13-2021)

Good IHC staining for Rat MMP13.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: rat knee joints

MMP13 Antibody Immunohistochemistry validation (1:100 dilution) in rat knee joints (Cat no:18165-1-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

clear wb band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP13 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:18165-1-AP)

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