MPG Polyclonal antibody

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• MPG Polyclonal antibody (11481-2-AP)

MPG Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11481-2-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

3 alkyladenine DNA glycosylase, 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG

• 

  WB analysis using 11481-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 11481-2-AP

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 11481-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, L02 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11481-2-AP targets MPG in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2034

  Product name: Recombinant human MPG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-293 aa of BC014991

  Sequence: MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA

• Full Name: N-methylpurine-DNA glycosylase
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014991
• Gene Symbol: MPG
• Gene ID (NCBI): 4350
• RRID: AB_2282144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29372
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].

Publications

What published studies show

The MPG Polyclonal antibody (Cat# 11481-2-AP) has 5 total citations. These appear in EMBO Reports, Reproductive Biomedicine Online, Journal of Oncology, Journal of Radiation Research, and Biomedicines. The cited research spans cellular senescence, reproductive biology and infertility, glioblastoma oncology, DNA base excision repair and radiation biology, and osteoporosis biomarker discovery. All cited applications were Western blot, with reactivity in human and mouse.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	WB	Reprod Biomed Online; Proteomic signature of menstrual blood mesenchymal stromal cells and their extracellular vesicles in women with unexplained infertility.; Authors - Raminta Vaiciuleviciute
human	WB	J Oncol; A Novel Pseudogene Methylation Signature to Predict Temozolomide Outcome in Non-G-CIMP Glioblastomas.; Authors - Bowen Li
mouse	WB	J Radiat Res; Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β.; Authors - Mizuki Yamamoto
		Biomedicines; Multi-Omics Analysis Identifies TTYH3 and MPG as Candidate Osteoporosis-Associated Biomarkers in Osteoblasts and Characterizes Spatial Heterogeneity of SPP1 in the Osteoporotic Bone Microenvironment.; Authors - Hengyi Diao