MPG Polyclonal antibody

MPG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11481-2-AP

Synonyms

3 alkyladenine DNA glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, MPG, N methylpurine DNA glycosylase, PIG11, PIG16

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Validation Data Gallery

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  WB analysis of HepG2 using 11481-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11481-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11481-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11481-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11481-2-AP

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug.

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  IHC staining of human prostate cancer using 11481-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

11481-2-AP targets MPG in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MPG fusion protein Ag2034
• Full Name: N-methylpurine-DNA glycosylase
• Calculated Molecular Weight: 293 aa, 32 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014991
• Gene Symbol: MPG
• Gene ID (NCBI): 4350
• RRID: AB_2282144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].

Publications

Species	Application	Title
human	WB	EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	WB	J Oncol; A Novel Pseudogene Methylation Signature to Predict Temozolomide Outcome in Non-G-CIMP Glioblastomas.; Authors - Bowen Li
mouse	WB	J Radiat Res; Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β.; Authors - Mizuki Yamamoto