MPHOSPH9 Recombinant antibody

• 

  WB analysis using 83397-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83397-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of A431 using 83397-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83397-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

MPHOSPH9 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83397-5-RR

Clone No.240182D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6

---

Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, NIH/3T3 cells, PC-12 cells, SH-SY5Y cells, mouse brain tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83397-5-RR targets MPHOSPH9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MPHOSPH9 fusion protein Ag22228
• Full Name: M-phase phosphoprotein 9
• Calculated Molecular Weight: 1031 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC130440
• Gene Symbol: MPHOSPH9
• Gene ID (NCBI): 10198
• RRID: AB_3671049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q99550
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).