|Positive WB detected in||DU 145 cells, human brain tissue, HeLa cells, Y79 cells|
|Positive IP detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
17710-1-AP targets MPP5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MPP5 fusion protein Ag12010|
|Full Name||membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)|
|Calculated molecular weight||675 aa, 77 kDa|
|Observed molecular weight||70-80 kDa|
|GenBank accession number||BC095485|
|Gene ID (NCBI)||64398|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.
Crumbs 2 prevents cortical abnormalities in mouse dorsal telencephalon.
Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation.
Microarray and Morphological Analysis of Early Postnatal CRB2 Mutant Retinas on a Pure C57BL/6J Genetic Background.
MPP3 regulates levels of PALS1 and adhesion between photoreceptors and Müller cells.
BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.
MPP3 is required for maintenance of the apical junctional complex, neuronal migration, and stratification in the developing cortex.