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MPP5 Polyclonal antibody

MPP5 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17710-1-AP

Synonyms

MAGUK p55 subfamily member 5, MPP5, PALS1



Tested Applications

Positive WB detected inY79 cells, human brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

17710-1-AP targets MPP5 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPP5 fusion protein Ag12010
Full Name membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
Calculated molecular weight 675 aa, 77 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC095485
Gene symbol MPP5
Gene ID (NCBI) 64398
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

Protocols

Product Specific Protocols
WB protocol for MPP5 antibody 17710-1-APDownload protocol
IP protocol for MPP5 antibody 17710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neurosci Res

Crumbs 2 prevents cortical abnormalities in mouse dorsal telencephalon.

Authors - Jacobus J Dudok
mouseWB,IF

Dev Biol

Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation.

Authors - Lucinda J Hughes
mouseIHC

PLoS One

Microarray and Morphological Analysis of Early Postnatal CRB2 Mutant Retinas on a Pure C57BL/6J Genetic Background.

Authors - Alves Celso Henrique CH
mouseIHC

Glia

MPP3 regulates levels of PALS1 and adhesion between photoreceptors and Müller cells.

Authors - Dudok Jacobus J JJ
mouseIF

PLoS Genet

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

Authors - Ying Hsu
mouseIHC

J Neurosci

MPP3 is required for maintenance of the apical junctional complex, neuronal migration, and stratification in the developing cortex.

Authors - Dudok Jacobus J JJ