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MPP5 Polyclonal antibody

MPP5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17710-1-AP

Synonyms

MAGUK p55 subfamily member 5, MPP5, PALS1



Tested Applications

Positive WB detected inDU 145 cells, human brain tissue, Y79 cells, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17710-1-AP targets MPP5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPP5 fusion protein Ag12010
Full Name membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
Calculated molecular weight 675 aa, 77 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC095485
Gene symbol MPP5
Gene ID (NCBI) 64398
RRIDAB_2282012
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

Protocols

Product Specific Protocols
WB protocol for MPP5 antibody 17710-1-APDownload protocol
IHC protocol for MPP5 antibody 17710-1-APDownload protocol
IP protocol for MPP5 antibody 17710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuron

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Authors - Divya Jayaraman
mouseIF

Neuron

Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.

Authors - Robert Beattie
humanWB,IP

Nat Commun

CD13 orients the apical-basal polarity axis necessary for lumen formation.

Authors - Li-Ting Wang
mouseIF

Dev Cell

Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm

Authors - Marissa L Gredler
mouseIF

Cell Death Differ

The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities.

Authors - Raffaele Iennaco
humanWB,IF

J Am Soc Nephrol

Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier.

Authors - Annika Möller-Kerutt