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- KD/KO Validated
MPP5 Polyclonal antibody
MPP5 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 17710-1-AP
|Positive WB detected in||DU 145 cells, human brain tissue, HeLa cells, Y79 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 5 publications below|
|IHC||See 6 publications below|
|IF||See 12 publications below|
|IP||See 1 publications below|
17710-1-AP targets MPP5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MPP5 fusion protein Ag12010|
|Full Name||membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)|
|Calculated molecular weight||675 aa, 77 kDa|
|Observed molecular weight||70-80 kDa|
|GenBank accession number||BC095485|
|Gene ID (NCBI)||64398|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.
|Product Specific Protocols|
|WB protocol for MPP5 antibody 17710-1-AP||Download protocol|
|IHC protocol for MPP5 antibody 17710-1-AP||Download protocol|
|IP protocol for MPP5 antibody 17710-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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