MRE11 Polyclonal antibody

MRE11 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10744-1-AP

Synonyms

ATLD, HNGS1, MRE11, MRE11 homolog 1, MRE11 homolog A, MRE11A, MRE11B



Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10744-1-AP targets MRE11 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRE11 fusion protein Ag1105
Full Name MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC005241
Gene symbol MRE11A
Gene ID (NCBI) 4361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRE11 antibody 10744-1-APDownload protocol
IHC protocol for MRE11 antibody 10744-1-APDownload protocol
IF protocol for MRE11 antibody 10744-1-APDownload protocol
IP protocol for MRE11 antibody 10744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Physiol

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Authors - C E Aiken
humanWB

Arch Biochem Biophys

MiR-34s negatively regulate homologous recombination through targeting RAD51.

Authors - Shuangjing Chen