MRE11 Polyclonal antibody
MRE11 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 10744-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, K-562 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
ChIP | See 1 publications below |
Product Information
10744-1-AP targets MRE11 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MRE11 fusion protein Ag1105 |
Full Name | MRE11 meiotic recombination 11 homolog A (S. cerevisiae) |
Calculated molecular weight | 81 kDa |
Observed molecular weight | 81 kDa |
GenBank accession number | BC005241 |
Gene symbol | MRE11A |
Gene ID (NCBI) | 4361 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MRE11 antibody 10744-1-AP | Download protocol |
IHC protocol for MRE11 antibody 10744-1-AP | Download protocol |
IF protocol for MRE11 antibody 10744-1-AP | Download protocol |
IP protocol for MRE11 antibody 10744-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin | ||
Oncogene TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex | ||
Cell Death Dis Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2. | ||
Nat Commun A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress | ||
J Physiol Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats. | ||
Arch Biochem Biophys MiR-34s negatively regulate homologous recombination through targeting RAD51. |