MRE11 Polyclonal antibody

MRE11 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10744-1-AP

Synonyms

ATLD, HNGS1, MRE11, MRE11 homolog 1, MRE11 homolog A, MRE11A, MRE11B

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Validation Data Gallery

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  WB analysis of A431 using 10744-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10744-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10744-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10744-1-AP

  IP Result of anti-MRE11A (IP:10744-1-AP, 4ug; Detection:10744-1-AP 1:600) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lymphoma using 10744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10744-1-AP (MRE11 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 10744-1-AP

  Immunofluorescent analysis of A431 cells using 10744-1-AP (MRE11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

10744-1-AP targets MRE11 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRE11 fusion protein Ag1105
• Full Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• Calculated molecular weight: 81 kDa
• Observed molecular weight: 81 kDa
• GenBank accession number: BC005241
• Gene symbol: MRE11A
• Gene ID (NCBI): 4361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB,IF	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
human	WB,IHC,ChIP	Cell Death Dis; Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2.; Authors - Xin Zhang
human	WB	Nat Commun; A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress; Authors - Wezley C. Griffin
rat	WB	J Physiol; Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.; Authors - C E Aiken
human	WB	Arch Biochem Biophys; MiR-34s negatively regulate homologous recombination through targeting RAD51.; Authors - Shuangjing Chen