MRPS18B Polyclonal antibody
MRPS18B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16139-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse liver tissue, rat heart tissue, HEK-293 cells, MCF-7 cells, PC-3 cells, SH-SY5Y cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 50 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Product Information
16139-1-AP targets MRPS18B in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MRPS18B fusion protein Ag9198 |
Full Name | mitochondrial ribosomal protein S18B |
Calculated Molecular Weight | 258 aa, 29 kDa |
Observed Molecular Weight | 29 kDa |
GenBank Accession Number | BC005373 |
Gene Symbol | MRPS18B |
Gene ID (NCBI) | 28973 |
RRID | AB_2146368 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
Protocols
Product Specific Protocols | |
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WB protocol for MRPS18B antibody 16139-1-AP | Download protocol |
IHC protocol for MRPS18B antibody 16139-1-AP | Download protocol |
IP protocol for MRPS18B antibody 16139-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Host Microbe Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition. | ||
Cell Metab The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. | ||
Nat Struct Mol Biol Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. | ||