MRPS18B Polyclonal antibody

MRPS18B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16139-1-AP

Synonyms

C6orf14, HSPC183, HumanS18a, MRP S18 2, MRP S18 b, MRPS18 2, Mrps18 b, MRPS18B, PTD017, S18amt, S18mt b



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16139-1-AP targets MRPS18B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS18B fusion protein Ag9198
Full Name mitochondrial ribosomal protein S18B
Calculated molecular weight 258 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC005373
Gene symbol MRPS18B
Gene ID (NCBI) 28973
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.

Protocols

Product Specific Protocols
WB protocol for MRPS18B antibody 16139-1-APDownload protocol
IHC protocol for MRPS18B antibody 16139-1-APDownload protocol
IF protocol for MRPS18B antibody 16139-1-APDownload protocol
IP protocol for MRPS18B antibody 16139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Authors - Uwe Richter
humanWB

Biochim Biophys Acta

Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.

Authors - Serre Valérie V
humanWB

Front Genet

RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Authors - Aurelio Reyes
mouseWB

Cell Rep

FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.

Authors - Sneha Shah
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
human

Acta Naturae

Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.

Authors - Shevchuk Z Z