MRPS18B Polyclonal antibody
MRPS18B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 16139-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat heart tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 39 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
16139-1-AP targets MRPS18B in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPS18B fusion protein Ag9198 |
| Full Name | mitochondrial ribosomal protein S18B |
| Calculated molecular weight | 258 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC005373 |
| Gene symbol | MRPS18B |
| Gene ID (NCBI) | 28973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPS18B antibody 16139-1-AP | Download protocol |
| IHC protocol for MRPS18B antibody 16139-1-AP | Download protocol |
| IF protocol for MRPS18B antibody 16139-1-AP | Download protocol |
| IP protocol for MRPS18B antibody 16139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Alliance Mitochondrial stress response triggered by defects in protein synthesis quality control. | ||
Biochim Biophys Acta Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency. | ||
Front Genet RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. | ||
Cell Rep FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism. | ||
PLoS Genet RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. | ||
