Name: MRPS18B Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

MRPS18B Antibody IHC human breast cancer tissue 16139-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

MRPS18B Antibody WB HeLa cells 16139-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MRPS18B Antibody IF HepG2 cells 16139-1-AP

Immunofluorescent analysis of HepG2 cells using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 andand Rhodamine-labeled goat anti-rabbit IgG (red).

MRPS18B Antibody IP HeLa cells 16139-1-AP

IP Result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse liver tissue, rat heart tissue

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human breast cancer tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HepG2 cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

MRPS18B fusion protein Ag9198

Full name:

mitochondrial ribosomal protein S18B

Calculated molecular weight:

258aa,29 kDa

Observed molecular weight:

29 kDa

GenBank accession number:

BC005373

Gene ID (NCBI):

28973

Gene symbol

MRPS18B

Synonyms

C6orf14, HSPC183, HumanS18a, MRP S18 2, MRP S18 b, MRPS18 2, Mrps18 b, MRPS18B, PTD017, S18amt, S18mt b

Background

MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.

Showing 1- [6] of [31] Publications

Author:		Wydro Mateusz M
Pubmed ID:		20144953
Journal:		Nucleic Acids Res
Title:		Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition.
Application:		WB

Species:		human

Author:		Dennerlein Sven S
Pubmed ID:		20604745
Journal:		Biochem J
Title:		Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.
Application:		WB

Species:		human

Author:		Kolanczyk Mateusz M
Pubmed ID:		21118999
Journal:		Mol Biol Cell
Title:		NOA1 is an essential GTPase required for mitochondrial protein synthesis.
Application:		WB

Species:		human

Author:		Surovtseva Yulia V YV
Pubmed ID:		22003127
Journal:		Proc Natl Acad Sci U S A
Title:		Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.
Application:		WB

Species:		human

Author:		Rorbach Joanna J
Pubmed ID:		22238376
Journal:		Nucleic Acids Res
Title:		C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial ribosome.
Application:		WB

Species:		human

Author:		Wanschers Bas F J BF
Pubmed ID:		22238375
Journal:		Nucleic Acids Res
Title:		C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.
Application:		WB

Species:		human

WB Figures

HeLa cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MRPS18B Antibody WB HepG2 cells 16139-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MRPS18B Antibody WB human liver tissue 16139-1-AP

human liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MRPS18B Antibody WB MCF-7 cells 16139-1-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MRPS18B Antibody WB mouse liver tissue 16139-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP(MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MRPS18B Antibody WB rat heart tissue 16139-1-AP

rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP( MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human breast cancer using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 40x lens)

MRPS18B Antibody IHC human liver tissue 16139-1-AP

Immunohistochemical of paraffin-embedded human liver using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human liver using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.

IF Figures

Immunofluorescent analysis of HepG2 cells using 16139-1-AP(MRPS18B antibody) at dilution of 1:50 andand Rhodamine-labeled goat anti-rabbit IgG (red).

MRPS18B Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 16139-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [31] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

MRPS18B Antibody 31 Publications

Rabbit Polyclonal| Catalog number: 16139-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background