Validation Data Gallery
|Positive WB detected in||A549 cells, mouse liver tissue, rat heart tissue, HEK-293 cells, MCF-7 cells, PC-3 cells, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16139-1-AP targets MRPS18B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MRPS18B fusion protein Ag9198|
|Full Name||mitochondrial ribosomal protein S18B|
|Calculated molecular weight||258 aa, 29 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC005373|
|Gene ID (NCBI)||28973|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
|Product Specific Protocols|
|WB protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IHC protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IF protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IP protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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