Validation Data Gallery
|Positive WB detected in||A549 cells, mouse liver tissue, rat heart tissue, HEK-293 cells, MCF-7 cells, PC-3 cells, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16139-1-AP targets MRPS18B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MRPS18B fusion protein Ag9198|
|Full Name||mitochondrial ribosomal protein S18B|
|Calculated molecular weight||258 aa, 29 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC005373|
|Gene ID (NCBI)||28973|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.
|Product Specific Protocols|
|WB protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IHC protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IF protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|IP protocol for MRPS18B antibody 16139-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Life Sci Alliance
Mitochondrial stress response triggered by defects in protein synthesis quality control.
Biochim Biophys Acta
Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.
FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.
RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.
Expression pattern of MRPS18 family genes in malignantly transformed b-cells.