CD20 Monoclonal antibody

CD20 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4A7G3

Cat no : 60271-1-Ig

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7



Tested Applications

Positive WB detected inRaji cells, Daudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, Raji cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:15000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60271-1-Ig is CD20 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002807
Gene symbol MS4A1
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD20 antibody 60271-1-IgDownload protocol
IHC protocol for CD20 antibody 60271-1-IgDownload protocol
IF protocol for CD20 antibody 60271-1-IgDownload protocol
FC protocol for CD20 antibody 60271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Int Immunopharmacol

Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer.

Authors - Huifang Liu
humanIHC

Front Cardiovasc Med

Integrating Bulk Transcriptome and Single-Cell RNA Sequencing Data Reveals the Landscape of the Immune Microenvironment in Thoracic Aortic Aneurysms.

Authors - Qunhui Wang
humanIHC

J Endocrinol Invest

The distinctive characteristics of the micro-vasculature and immune cell infiltration in cystic pancreatic neuroendocrine tumors.

Authors - H Gao
humanIHC

Front Genet

RNA-Binding Protein IGF2BP1 Associated With Prognosis and Immunotherapy Response in Lung Adenocarcinoma.

Authors - JinFeng Liu
humanIHC

Clin Exp Med

Altered peripheral helper T cells in peripheral blood and muscle tissue of the patients with dermatomyositis.

Authors - Xiaoyu Hou
humanIHC

World J Surg Oncol

The downregulation of NCXs is positively correlated with the prognosis of stage II-IV colon cancer.

Authors - Zhixiu Xia