|Positive WB detected in||Raji cells, Daudi cells, Ramos cells|
|Positive IHC detected in||human tonsillitis tissue, human appendicitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:15000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60271-1-Ig is CD20 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||CD20 fusion protein Ag17309|
|Full Name||membrane-spanning 4-domains, subfamily A, member 1|
|Calculated molecular weight||297 aa, 33 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002807|
|Gene ID (NCBI)||931|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
|Product Specific Protocols|
|WB protocol for CD20 antibody 60271-1-Ig||Download protocol|
|IHC protocol for CD20 antibody 60271-1-Ig||Download protocol|
|IF protocol for CD20 antibody 60271-1-Ig||Download protocol|
|FC protocol for CD20 antibody 60271-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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