CoraLite® Plus 647-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL647-60271

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7



Tested Applications

Positive IF detected inhuman tonsillitis tissue, human lung cancer tissue
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60271 targets CD20 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated Molecular Weight 297 aa, 33 kDa
GenBank Accession NumberBC002807
Gene Symbol MS4A1
Gene ID (NCBI) 931
RRIDAB_2883651
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD20 antibody CL647-60271Download protocol
FC protocol for CL Plus 647 CD20 antibody CL647-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols