CoraLite®647-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL647-60271

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRaji cells
For other applications, we recommend the unconjugated version of this antibody, 60271-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL647-60271 is CD20 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol MS4A1
Gene ID (NCBI) 931
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Protocols

Product Specific Protocols
IF protocol for CL647 CD20 antibody CL647-60271Download protocol
FC protocol for CL647 CD20 antibody CL647-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols